AB204124

Anti-FUT8 antibody

Name: Anti-FUT8 antibody (ab204124) | Abcam
Brand: Abcam Limited
SKU: AB204124
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal FUT8 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human FUT8 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Alpha1-6FucT, Fucosyltransferase 8, GDP-fucose--glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase, FUT8

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT8 antibody (AB204124)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT8 antibody (AB204124)

Immunohistochemical analysis of formalin-fixed and paraffin embedded rat brain tissue labeling FUT8 with ab204124 at 1/200 followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human FUT8 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9BYC5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using fluorescent probe the recommended dilution range is 1/50 - 1/200.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FUT8 also known as alpha-(16)-fucosyltransferase represents an important enzyme responsible for transferring fucose from GDP-fucose to the innermost N-acetylglucosamine of N-glycans producing core fucosylation. The molecular mass of FUT8 is approximately 60 kDa. It expresses ubiquitously across various tissues with elevated levels seen in the liver lung and placenta. Researchers frequently study FUT8 due to its significance in glycosylation processes.

Biological function summary

FUT8 impacts numerous cellular activities by modulating glycan branching on glycoproteins. It functions independently and is not directly part of a larger protein complex. FUT8's activity influences cell-cell interactions signaling pathways and receptor functions. It plays an important part in maintaining cellular homeostasis and influences immune responses and cell growth as affected by glycosylation patterns on the cell surface and extracellular matrix.

Pathways

FUT8 is a critical component of the N-glycan biosynthesis pathway where it regulates the addition of fucose to glycans. This process affects protein folding and stability. The enzyme interacts with important glycoproteins like EGFR and integrins impacting signaling pathways such as the Wnt and TGF-beta pathways. Altered FUT8 activity can modify pathways and relationships with other proteins changing cellular responses.

FUT8 has connections to cancers and liver fibrosis due to its role in modifying glycans and subsequently influencing cellular communication and adhesion. Abnormal core fucosylation affects proteins like alpha-fetoprotein in liver cancer which might serve as a biomarker. Furthermore changes in FUT8 activity and expression could relate to epidermal growth factor receptor (EGFR) signaling disruptions promoting tumor proliferation. Scientists consider FUT8 a potential therapeutic target in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.

See full target information FUT8

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Oncology 32:200783 PubMed38595983

2024

Conditionally replicative adenovirus as a therapy for malignant peripheral nerve sheath tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Nikrad,Robert T Galvin,Mackenzie M Sheehy,Ethan L Novacek,Kari L Jacobsen,Stanislas M A S Corbière,Pauline J Beckmann,Tyler A Jubenville,Masato Yamamoto,David A Largaespada

Immunity, inflammation and disease 10:e686 PubMed36039648

2022

Positive association of serum FUT8 activity with renal tubulointerstitial injury in IgA nephropathy patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Yang,Long-Kai Li,Hui He,Xia-Nan Guo,Xue-Feng Yuan,Zhi-Tong Li,Wei-Dong Wang,Biao-Jie Qin,Xiang-Ning Du,Xu Zhang,Shu-Ni Chen,Hong-Li Lin

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 55:113-122 PubMed35504805

2022

HOTAIR modulates hepatocellular carcinoma progression by activating FUT8/core-fucosylated Hsp90/MUC1/STAT3 feedback loop via JAK1/STAT3 cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Yanru Guo,Bing Liu,Tong Huang,Xia Qi,Shijun Li

Biochimica et biophysica acta. General subjects 1864:129668 PubMed32553689

2020

Dynamic alterations in serum IgG N-glycan profiles in the development of colitis-associated colon Cancer in mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Gu,Jing Han,Xin Liu,Yiqing Pan,Xiaoyan Xu,Jichen Sha,Shifang Ren,Jianxin Gu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FUT8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT8 antibody (AB204124)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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