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Rabbit Polyclonal FUT9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FUT9 aa 150 to C-terminus.


Images

Western blot - Anti-FUT9 antibody (AB176794), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT9 antibody (AB176794), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human FUT9 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y231

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Cow
Predicted
Predicted
Dog
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow, Dog, Chimpanzee

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow, Dog, Chimpanzee

Dilution info

-

Notes

-

Target data

Function

Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, beta-D-Gal-(1->4)-beta-D-GlcNAc-) glycan attached to glycolipids and N- or O-linked glycoproteins. Fucosylates distal type 2 LacNAc and its fucosylated (H-type 2 LacNAc) and sialylated (sialyl-type 2 LacNAc) derivatives to form Lewis x (Lex) (CD15) and Lewis y (Ley) antigenic epitopes involved in cell adhesion and differentiation (PubMed:10386598, PubMed:10622713, PubMed:11278338, PubMed:12107078, PubMed:16282604, PubMed:17335083, PubMed:18395013, PubMed:23192350, PubMed:23263199, PubMed:29593094, PubMed:37202521). Generates Lex epitopes in the brain, presumably playing a role in the maintenance of neuronal stemness and neurite outgrowth in progenitor neural cells (By similarity) (PubMed:17335083, PubMed:23000574). Fucosylates the internal type 2 LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:23192350). Can also modify milk oligosaccharides, in particular type 2 tetrasaccharide LNnT (PubMed:37202521).

Alternative names

Recommended products

Rabbit Polyclonal FUT9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FUT9 aa 150 to C-terminus.

Key facts

Isotype

IgG

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human FUT9 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y231
Purification technique

Affinity purification Protein A

Reconstitution

Reconstitute in 200 µl sterile water.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The fucosyltransferase 9 (FUT9) is an enzyme that transfers fucose a hexose deoxy sugar to N-acetylglucosamine which is part of the process of glycosylation. Also known as FT9 this enzyme weighs approximately 44 kDa. It is expressed in many tissues but has higher levels in the brain. FUT9 functions to add fucose residues in the synthesis of Lewis X (LeX) antigens which are important for cell-cell interactions.

Biological function summary

The enzyme contributes to the synthesis of glycoproteins and glycolipids impacting cellular adhesion and immune response. FUT9 does not typically join with protein complexes but it plays a significant role in forming the structures essential for developmental processes. The glycan structures generated by FUT9 involve in developmental decisions and the maturation of specific cellular functions particularly in the nervous system.

Pathways

FUT9 participates actively in the N-glycan biosynthesis pathway and specifically influences the creation of the Lewis X antigen. This antigen plays a role in pathways related to leukocyte migration and neural development. In these pathways FUT9 interacts indirectly with adhesion molecules and lectins which recognize and bind to specific sugar structures like LeX affecting downstream signaling and cell movement.

Associated diseases and disorders

FUT9 relates to certain types of cancer and neurological diseases. Altered fucosylation patterns mediated by FUT9 associate with tumor metastasis and cancer progression. In the context of neurological disorders irregular FUT9 activity correlates with conditions like Alzheimer's where cell adhesion molecules affected by FUT9-mediated fucosylation undergo alterations. This enzyme's interaction with adhesion proteins can influence disease state and severity in these cases.

Product promise

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2 product images

  • Western blot - Anti-FUT9 antibody (ab176794), expandable thumbnail

    Western blot - Anti-FUT9 antibody (ab176794)

    All lanes: Western blot - Anti-FUT9 antibody (ab176794) at 1/500 dilution

    All lanes: K562 cell lysate

    Predicted band size: 100 kDa, 118 kDa, 120 kDa, 153 kDa, 17 kDa, 29 kDa, 42 kDa, 43 kDa, 48 kDa, 52 kDa, 54 kDa, 57 kDa, 70 kDa

    Observed band size: 53 kDa, 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT9 antibody (ab176794), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT9 antibody (ab176794)

    Immunohistochemical staining of formalin fixed, paraffin embedded Human fetal stomach tissue labeling FUT9 with ab176794 at 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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