Rabbit Polyclonal FUT9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FUT9 aa 150 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Lyophilized
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Predicted | Predicted |
Rat | Predicted | Predicted |
Chimpanzee | Predicted | Predicted |
Cow | Predicted | Predicted |
Dog | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Dog, Chimpanzee | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Dog, Chimpanzee | Dilution info - | Notes - |
Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, beta-D-Gal-(1->4)-beta-D-GlcNAc-) glycan attached to glycolipids and N- or O-linked glycoproteins. Fucosylates distal type 2 LacNAc and its fucosylated (H-type 2 LacNAc) and sialylated (sialyl-type 2 LacNAc) derivatives to form Lewis x (Lex) (CD15) and Lewis y (Ley) antigenic epitopes involved in cell adhesion and differentiation (PubMed:10386598, PubMed:10622713, PubMed:11278338, PubMed:12107078, PubMed:16282604, PubMed:17335083, PubMed:18395013, PubMed:23192350, PubMed:23263199, PubMed:29593094, PubMed:37202521). Generates Lex epitopes in the brain, presumably playing a role in the maintenance of neuronal stemness and neurite outgrowth in progenitor neural cells (By similarity) (PubMed:17335083, PubMed:23000574). Fucosylates the internal type 2 LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:23192350). Can also modify milk oligosaccharides, in particular type 2 tetrasaccharide LNnT (PubMed:37202521).
4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9, Fucosyltransferase 9, Fucosyltransferase IX, Galactoside 3-L-fucosyltransferase, Fuc-TIX, FucT-IX, FUT9
Rabbit Polyclonal FUT9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FUT9 aa 150 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Lyophilized
Polyclonal
Affinity purification Protein A
Reconstitute in 200 µl sterile water.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The fucosyltransferase 9 (FUT9) is an enzyme that transfers fucose a hexose deoxy sugar to N-acetylglucosamine which is part of the process of glycosylation. Also known as FT9 this enzyme weighs approximately 44 kDa. It is expressed in many tissues but has higher levels in the brain. FUT9 functions to add fucose residues in the synthesis of Lewis X (LeX) antigens which are important for cell-cell interactions.
The enzyme contributes to the synthesis of glycoproteins and glycolipids impacting cellular adhesion and immune response. FUT9 does not typically join with protein complexes but it plays a significant role in forming the structures essential for developmental processes. The glycan structures generated by FUT9 involve in developmental decisions and the maturation of specific cellular functions particularly in the nervous system.
FUT9 participates actively in the N-glycan biosynthesis pathway and specifically influences the creation of the Lewis X antigen. This antigen plays a role in pathways related to leukocyte migration and neural development. In these pathways FUT9 interacts indirectly with adhesion molecules and lectins which recognize and bind to specific sugar structures like LeX affecting downstream signaling and cell movement.
FUT9 relates to certain types of cancer and neurological diseases. Altered fucosylation patterns mediated by FUT9 associate with tumor metastasis and cancer progression. In the context of neurological disorders irregular FUT9 activity correlates with conditions like Alzheimer's where cell adhesion molecules affected by FUT9-mediated fucosylation undergo alterations. This enzyme's interaction with adhesion proteins can influence disease state and severity in these cases.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-FUT9 antibody (ab176794) at 1/500 dilution
All lanes: K562 cell lysate
Predicted band size: 100 kDa, 118 kDa, 120 kDa, 153 kDa, 17 kDa, 29 kDa, 42 kDa, 43 kDa, 48 kDa, 52 kDa, 54 kDa, 57 kDa, 70 kDa
Observed band size: 53 kDa, 54 kDa
Immunohistochemical staining of formalin fixed, paraffin embedded Human fetal stomach tissue labeling FUT9 with ab176794 at 1/100 dilution.
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