AB111842

Anti-FUZ antibody

Name: Anti-FUZ antibody (ab111842) | Abcam
Brand: Abcam Limited
SKU: AB111842
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal FUZ antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FUZ aa 50-350.

View Alternative Names

FY, FUZ, Protein fuzzy homolog

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (AB111842)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (AB111842)

ab111842, at 1/500 dilution, staining FUZ in Formalin-fixed, Paraffin-embedded Human Gastric carcinoma tissue by Immunohistochemistry.

Unknown

Western blot - Anti-FUZ antibody (AB111842)

10% SDS PAGE

All lanes:

Western blot - Anti-FUZ antibody (ab111842) at 1/1000 dilution

All lanes:

HCT116 cell line lysate at 30 µg

Predicted band size: 35 kDa,43 kDa,46 kDa,50 kDa,78 kDa

Observed band size: 50 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (AB111842)

ab111842, at 1/500 dilution, staining FUZ in Formalin-fixed, Paraffin-embedded RT2 xenograft tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FUZ aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9BT04

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab111842 is purified by antigen-affinity chromatography.

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FUZ protein also known as fuzzy homolog (Drosophila) functions mechanically as a planar cell polarity protein. Its mass is approximately 51.5 kDa. FUZ expresses in various tissues significantly within the neural tissues. This protein plays an important role in the regulation of cell shape and movement especially in neural crest cells and ependymal motile cilia.

Biological function summary

The FUZ protein participates in complex processes involved in cellular organization and signal transduction. FUZ acts within the cytoskeletal network influencing the cytoskeleton dynamics necessary for cell polarity and orientation. It is not known to be a part of a distinct protein complex but its role connects deeply to the modulation of intracellular signaling pathways necessary for embryonic development and tissue morphogenesis.

Pathways

The FUZ protein engages in neural crest cell pathway and planar cell polarity (PCP) signaling pathway. Within PCP it associates with proteins like Dishevelled (DVL) and Prickle (PRICKLE) to direct cell orientation and polarity. In neural crest formation its regulation impacts proper migration and differentiation indicating its linkage to other cellular proteins such as cadherins and integrins that mediate cell-cell communication and adhesion.

FUZ disruptions correlate with neural tube defects and ciliopathies. Improper expression or mutation in the FUZ gene associates with congenital conditions affecting the neural tube closure. Additionally FUZ contributes to the pathogenesis of ciliopathies where it affects ependymal cilia movement. It connects with proteins like KIF3A and other intraflagellar transport proteins in this context highlighting its relevance to disorders involving ciliary dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies. May regulate the morphogenesis of hair follicles which depends on functional primary cilia. Binds phosphatidylinositol 3-phosphate with highest affinity, followed by phosphatidylinositol 4-phosphate and phosphatidylinositol 5-phosphate (By similarity).

See full target information FUZ

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 13:2419-2429 PubMed35711836

2022

Fuzzy Planar Cell Polarity Gene (FUZ) Promtes Cell Glycolysis, Migration, and Invasion in Non-small Cell Lung Cancer via the Phosphoinositide 3-Kinase/Protein Kinase B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Wang,Hui Zhang,Bulin Du,Xuena Li,Yaming Li

Frontiers in oncology 11:683915 PubMed34976784

2021

Development and Validation of an Immune-Related Signature for the Prediction of Recurrence Risk of Patients With Laryngeal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhang,Xudong Zhao,Jin Wang,Wenyue Ji

EMBO reports 19: PubMed30026307

2018

Planar cell polarity gene triggers apoptosis in neurodegenerative disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefan Stephen Chen,Li Li,Shaohong Peng,Francis M Chen,Qian Zhang,Ying An,Xiao Lin,Wen Li,Alex Chun Koon,Ting-Fung Chan,Kwok-Fai Lau,Jacky Chi Ki Ngo,Wing Tak Wong,Kin Ming Kwan,Ho Yin Edwin Chan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FUZ antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (AB111842)

Western blot - Anti-FUZ antibody (AB111842)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (AB111842)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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