Rabbit Polyclonal FUZ antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FUZ aa 50-350.
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- Journal of Cancer 13:2419-24292022Fuzzy Planar Cell Polarity Gene (FUZ) Promtes Cell Glycolysis, Migration, and Invasion in Non-small Cell Lung Cancer via the Phosphoinositide 3-Kinase/Protein Kinase B Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Wang,Hui Zhang,Bulin Du,Xuena Li,Yaming LiPubMed 35711836
- Frontiers in oncology 11:6839152021Development and Validation of an Immune-Related Signature for the Prediction of Recurrence Risk of Patients With Laryngeal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Zhang,Xudong Zhao,Jin Wang,Wenyue JiPubMed 34976784
- EMBO reports 19:2018Planar cell polarity gene triggers apoptosis in neurodegenerative disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhefan Stephen Chen et. al.PubMed 30026307
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FUZ aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BT04
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/500 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
Species Human | Dilution info 1/100 - 1/500 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
Associated Products
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Target data
Function
Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies. May regulate the morphogenesis of hair follicles which depends on functional primary cilia. Binds phosphatidylinositol 3-phosphate with highest affinity, followed by phosphatidylinositol 4-phosphate and phosphatidylinositol 5-phosphate (By similarity).
Alternative names
FY, FUZ, Protein fuzzy homolog
Recommended products
Rabbit Polyclonal FUZ antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FUZ aa 50-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FUZ aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BT04
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab111842 is purified by antigen-affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FUZ protein also known as fuzzy homolog (Drosophila) functions mechanically as a planar cell polarity protein. Its mass is approximately 51.5 kDa. FUZ expresses in various tissues significantly within the neural tissues. This protein plays an important role in the regulation of cell shape and movement especially in neural crest cells and ependymal motile cilia.
- Biological function summary
The FUZ protein participates in complex processes involved in cellular organization and signal transduction. FUZ acts within the cytoskeletal network influencing the cytoskeleton dynamics necessary for cell polarity and orientation. It is not known to be a part of a distinct protein complex but its role connects deeply to the modulation of intracellular signaling pathways necessary for embryonic development and tissue morphogenesis.
- Pathways
The FUZ protein engages in neural crest cell pathway and planar cell polarity (PCP) signaling pathway. Within PCP it associates with proteins like Dishevelled (DVL) and Prickle (PRICKLE) to direct cell orientation and polarity. In neural crest formation its regulation impacts proper migration and differentiation indicating its linkage to other cellular proteins such as cadherins and integrins that mediate cell-cell communication and adhesion.
- Associated diseases and disorders
FUZ disruptions correlate with neural tube defects and ciliopathies. Improper expression or mutation in the FUZ gene associates with congenital conditions affecting the neural tube closure. Additionally FUZ contributes to the pathogenesis of ciliopathies where it affects ependymal cilia movement. It connects with proteins like KIF3A and other intraflagellar transport proteins in this context highlighting its relevance to disorders involving ciliary dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-FUZ antibody (ab111842)
10% SDS PAGE
All lanes: Western blot - Anti-FUZ antibody (ab111842) at 1/1000 dilution
All lanes: HCT116 cell line lysate at 30 µg
Predicted band size: 35 kDa, 43 kDa, 46 kDa, 50 kDa, 78 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (ab111842)
ab111842, at 1/500 dilution, staining FUZ in Formalin-fixed, Paraffin-embedded Human Gastric carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (ab111842)
ab111842, at 1/500 dilution, staining FUZ in Formalin-fixed, Paraffin-embedded RT2 xenograft tissue by Immunohistochemistry.
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