Rabbit Polyclonal FUZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FUZ aa 300 to C-terminus.
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Publications
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- Life sciences 197:91-1002018In vitro study of FUZ as a novel potential therapeutic target in non-small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinwei He et. al.PubMed 29421438
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FUZ aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BT04
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Associated Products
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Target data
Function
Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies. May regulate the morphogenesis of hair follicles which depends on functional primary cilia. Binds phosphatidylinositol 3-phosphate with highest affinity, followed by phosphatidylinositol 4-phosphate and phosphatidylinositol 5-phosphate (By similarity).
Alternative names
FY, FUZ, Protein fuzzy homolog
Recommended products
Rabbit Polyclonal FUZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FUZ aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FUZ aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BT04
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FUZ protein also known as fuzzy homolog (Drosophila) functions mechanically as a planar cell polarity protein. Its mass is approximately 51.5 kDa. FUZ expresses in various tissues significantly within the neural tissues. This protein plays an important role in the regulation of cell shape and movement especially in neural crest cells and ependymal motile cilia.
- Biological function summary
The FUZ protein participates in complex processes involved in cellular organization and signal transduction. FUZ acts within the cytoskeletal network influencing the cytoskeleton dynamics necessary for cell polarity and orientation. It is not known to be a part of a distinct protein complex but its role connects deeply to the modulation of intracellular signaling pathways necessary for embryonic development and tissue morphogenesis.
- Pathways
The FUZ protein engages in neural crest cell pathway and planar cell polarity (PCP) signaling pathway. Within PCP it associates with proteins like Dishevelled (DVL) and Prickle (PRICKLE) to direct cell orientation and polarity. In neural crest formation its regulation impacts proper migration and differentiation indicating its linkage to other cellular proteins such as cadherins and integrins that mediate cell-cell communication and adhesion.
- Associated diseases and disorders
FUZ disruptions correlate with neural tube defects and ciliopathies. Improper expression or mutation in the FUZ gene associates with congenital conditions affecting the neural tube closure. Additionally FUZ contributes to the pathogenesis of ciliopathies where it affects ependymal cilia movement. It connects with proteins like KIF3A and other intraflagellar transport proteins in this context highlighting its relevance to disorders involving ciliary dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-FUZ antibody (ab122742)
Immunocytochemistry/immunofluorescence analysis of HEK293 cells labelling FUZ with ab122742 shows positive staining in the cytoplasm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (ab122742)
ab122742 at 1/50 dilution staining FUZ in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry.
Western blot - Anti-FUZ antibody (ab122742)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.All lanes: Western blot - Anti-FUZ antibody (ab122742)
Predicted band size: 46 kDa
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