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AB122742

Anti-FUZ antibody

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(1 Publication)

Rabbit Polyclonal FUZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FUZ aa 300 to C-terminus.

View Alternative Names

FY, FUZ, Protein fuzzy homolog

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FUZ antibody (AB122742)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FUZ antibody (AB122742)

Immunocytochemistry/immunofluorescence analysis of HEK293 cells labelling FUZ with ab122742 shows positive staining in the cytoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (AB122742)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (AB122742)

ab122742 at 1/50 dilution staining FUZ in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry.

Western blot - Anti-FUZ antibody (AB122742)
  • WB

Unknown

Western blot - Anti-FUZ antibody (AB122742)

Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

All lanes:

Western blot - Anti-FUZ antibody (ab122742)

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human FUZ aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BT04

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FUZ protein also known as fuzzy homolog (Drosophila) functions mechanically as a planar cell polarity protein. Its mass is approximately 51.5 kDa. FUZ expresses in various tissues significantly within the neural tissues. This protein plays an important role in the regulation of cell shape and movement especially in neural crest cells and ependymal motile cilia.
Biological function summary

The FUZ protein participates in complex processes involved in cellular organization and signal transduction. FUZ acts within the cytoskeletal network influencing the cytoskeleton dynamics necessary for cell polarity and orientation. It is not known to be a part of a distinct protein complex but its role connects deeply to the modulation of intracellular signaling pathways necessary for embryonic development and tissue morphogenesis.

Pathways

The FUZ protein engages in neural crest cell pathway and planar cell polarity (PCP) signaling pathway. Within PCP it associates with proteins like Dishevelled (DVL) and Prickle (PRICKLE) to direct cell orientation and polarity. In neural crest formation its regulation impacts proper migration and differentiation indicating its linkage to other cellular proteins such as cadherins and integrins that mediate cell-cell communication and adhesion.

FUZ disruptions correlate with neural tube defects and ciliopathies. Improper expression or mutation in the FUZ gene associates with congenital conditions affecting the neural tube closure. Additionally FUZ contributes to the pathogenesis of ciliopathies where it affects ependymal cilia movement. It connects with proteins like KIF3A and other intraflagellar transport proteins in this context highlighting its relevance to disorders involving ciliary dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies. May regulate the morphogenesis of hair follicles which depends on functional primary cilia. Binds phosphatidylinositol 3-phosphate with highest affinity, followed by phosphatidylinositol 4-phosphate and phosphatidylinositol 5-phosphate (By similarity).
See full target information FUZ
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life sciences 197:91-100 PubMed29421438

2018

In vitro study of FUZ as a novel potential therapeutic target in non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minwei He,Kangqi Li,Chuanfei Yu,Bingfeng Lv,Ning Zhao,Jinhai Deng,Lulu Cao,He Huang,Ang Yin,Taiping Shi,Lu Wang
View all publications
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