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Rabbit Recombinant Monoclonal FXI antibody. Suitable for WB, Dot and reacts with Human, Recombinant fragment - Human samples.

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Images

Dot Blot - Anti-FXI antibody [EPR29204-87] (AB323450), expandable thumbnail
  • Western blot - Anti-FXI antibody [EPR29204-87] (AB323450), expandable thumbnail
  • Western blot - Anti-FXI antibody [EPR29204-87] (AB323450), expandable thumbnail
  • Western blot - Anti-FXI antibody [EPR29204-87] (AB323450), expandable thumbnail
  • Western blot - Anti-FXI antibody [EPR29204-87] (AB323450), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PDot
Human
Tested
Not recommended
Expected
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.

Alternative names

Coagulation factor XI, FXI, Plasma thromboplastin antecedent, PTA, F11

Rabbit Recombinant Monoclonal FXI antibody. Suitable for WB, Dot and reacts with Human, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29204-87
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Dot Blot - Anti-FXI antibody [EPR29204-87] (ab323450), expandable thumbnail

    Dot Blot - Anti-FXI antibody [EPR29204-87] (ab323450)

    Dot blot analysis of FXI using ab323450 at 1000 (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: LIF-tagged Human FXI light chain fragment

    Lane2: LIF-tagged Human FXI heavy chain fragment

    Lane3: LIF-tagged Human KLKB1 fragment

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human FX1 heavy chain and Human KLKB1.

    Total protein control: Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) (Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) ab244795) (1:1000).

    All lanes: Dot Blot - Anti-FXI antibody [EPR29204-87] (ab323450) at 1/1000 dilution

    Lane 1: LIF-tagged Human FXI light chain fragment

    Lane 2: LIF-tagged Human FXI heavy chain fragment

    Lane 3: LIF-tagged Human KLKB1 fragment

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-FXI antibody [EPR29204-87] (ab323450), expandable thumbnail

    Western blot - Anti-FXI antibody [EPR29204-87] (ab323450)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature. The antibody detects the pro (XI) and active form (XIa) of FXI. PMID: 23515926, PMID: 23515926.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FXI antibody [EPR29204-87] (ab323450) at 1/1000 dilution

    Lane 1: Untreated HepG2 whole cell lysate at 80 µg

    Lane 2: HepG2 treated with 300ng/ml BFA for 18 hours whole cell lysate at 80 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 30-37 kDa, 70-75 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-FXI antibody [EPR29204-87] (ab323450), expandable thumbnail

    Western blot - Anti-FXI antibody [EPR29204-87] (ab323450)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    FXI is a glycoprotein of approximately 30-37, 70-75 kDa and is detected at a slightly lower molecular weight following N-glycosidase F (PNGase F) treatment.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29223926).

    For deglycosylation experiments, cells were incubated at 100°C for 10 mins, followed by incubation at 37°C for 1hr with or without N-glycosidase F (PNGase F).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FXI antibody [EPR29204-87] (ab323450) at 1/1000 dilution

    Lane 1: Untreated HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 80 µg

    Lane 2: HepG2 whole cell lysate treated with Peptide: N-glycosidase F (PNGase F) at 80 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 30-37 kDa, 70-75 kDa, 36 kDa

    Exposure time: 70s

  • Western blot - Anti-FXI antibody [EPR29204-87] (ab323450), expandable thumbnail

    Western blot - Anti-FXI antibody [EPR29204-87] (ab323450)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HeLa, MCF7.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29223926).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FXI antibody [EPR29204-87] (ab323450) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 80 µg

    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 80 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 30-37 kDa, 70-75 kDa, 36 kDa

    Exposure time: 70s

  • Western blot - Anti-FXI antibody [EPR29204-87] (ab323450), expandable thumbnail

    Western blot - Anti-FXI antibody [EPR29204-87] (ab323450)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29223926).

    The identity of the band higher 250 kDa (in lanes 1-2) is unknown.

    All lanes: Western blot - Anti-FXI antibody [EPR29204-87] (ab323450) at 1/1000 dilution

    Lane 1: Human plasma lysate at 20 µg

    Lane 2: Human serum lysate at 20 µg

    Lane 3: Human liver tissue lysate at 80 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 30-37 kDa, 70-75 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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