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AB320692

Anti-FXI antibody [EPR29297-66] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal FXI antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Coagulation factor XI, FXI, Plasma thromboplastin antecedent, PTA, F11

1 Images
Sandwich ELISA - Anti-FXI antibody [EPR29297-66] - BSA and Azide free (Detector) (AB320692)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-FXI antibody [EPR29297-66] - BSA and Azide free (Detector) (AB320692)

Sandwich ELISA of ab320690 with the capture antibody ab320691 dilution at 2 ug/mL and detector antibody ab320692 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29297-66

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with human Plasma Kallikrein 1B.

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Reactivity data

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Product details

ab320692 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human FXI. The recommended pair for sandwich ELISA is:

Capture: ab320691, Human FXI Capture Antibody (unconjugated)

Detector: ab320692, Human FXI Detector Antibody (unconjugated)

The reference range value is 19.53-1250 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XI (FXI) also known as Factor 11 or Factor Eleven is an important protein in the human body involved in blood coagulation. It has a molecular mass of approximately 160 kDa and is primarily expressed in the liver. Factor XI circulates in the blood as a zymogen an inactive enzyme precursor until it is activated during the coagulation process. Its activation is a critical step in the chain of events leading to blood clot formation. FXI can be measured using assays such as ELISA and it is often used in laboratory settings to study coagulation mechanics.
Biological function summary

FXI plays a significant role in the intrinsic pathway of the coagulation cascade. It forms a complex with high-molecular-weight kininogen and prekallikrein on the surface of activated platelets ensuring that the coagulation process proceeds efficiently. Upon activation by Factor XIIa FXI itself becomes an active serine protease known as FXIa which then converts Factor IX into its active form Factor IXa. This step highlights the importance of FXI in amplifying the coagulation process ensuring rapid and effective hemostasis.

Pathways

Factor XI is deeply integrated into the intrinsic coagulation cascade. It works alongside other proteins such as Factor XII and Factor IX to facilitate the formation of a blood clot. The intrinsic pathway is essential for maintaining hemostasis particularly in response to internal injuries within the blood vessels. Its interaction with these proteins allows for a powerful amplification of the clotting signal ensuring a robust clot formation and effectively sealing vascular injuries.

Dysfunction or deficiency in FXI can lead to bleeding disorders such as Hemophilia C although this is less common than Hemophilia A or B. Interestingly elevated levels of FXI are sometimes associated with an increased risk of thrombotic conditions such as deep vein thrombosis or stroke. In these conditions FXI interacts with other coagulation factors like Factor IX and Factor XII highlighting its dual role in both bleeding and clotting disorders. Understanding FXI's role provides insight into therapeutic targets for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
See full target information F11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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