AB168852

Anti-FXR2 antibody

Name: Anti-FXR2 antibody (ab168852) | Abcam
Brand: Abcam Limited
SKU: AB168852
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal FXR2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FXR2 aa 600-650.

View Alternative Names

FMR1L2, FXR2, RNA-binding protein FXR2, FXR2P, FMR1 autosomal homolog 2

2 Images

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Immunoprecipitation - Anti-FXR2 antibody (AB168852)

Detection of FXR2 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168852 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-FXR2 antibody (ab168852)

Predicted band size: 74 kDa

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Unknown

Western blot - Anti-FXR2 antibody (AB168852)

All lanes:

Western blot - Anti-FXR2 antibody (ab168852) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 74 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human FXR2 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

P51116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab168852 was affinity purified using an epitope specific to FXR2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FXR2 also known as Fragile X Mental Retardation Syndrome-Related Protein 2 is part of the fragile X-related family of proteins. It has a molecular mass of approximately 78 kDa. This protein is expressed in various tissues with higher levels in the brain and testes. FXR2 acts primarily as an RNA-binding protein and has functional RNA transport and translational repression roles. The protein contains several motifs including a KH domain and RGG boxes which aid its interaction with RNA.

Biological function summary

FXR2 is a part of a larger complex that includes homologous proteins like FMRP and FXR1. These proteins cooperate in the regulation of mRNA dynamics in neurons thereby playing essential roles in synaptic function and plasticity. They affect the translation of specific mRNAs and modulate the structure of dendritic spines. The FXR2 protein is important in the proper development of neurological architecture and cognitive processes.

Pathways

FXR2 participates in the mTOR signaling pathway and the regulation of dendritic translation. The mTOR pathway is vital for cell growth and protein synthesis critical in neural activity and development. FXR2 works alongside related proteins such as FMR1 and FXR1 within these pathways and these interactions modulate ribosome biogenesis and activity. FXR2 interactions importantly facilitate enhanced responses to synaptic activities.

FXR2 influences conditions like Fragile X syndrome and autism spectrum disorders. Aberrations in FXR2 expression or function disrupt neuronal development and synaptic plasticity and are linked to cognitive impairments. FXR2 shares close connections with proteins FMR1 and CYFIP1 within these disorders suggesting a broader network of protein interactions critical for normal neurological function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for adult hippocampal neurogenesis (By similarity). Specifically binds to AU-rich elements (AREs) in the 3'-UTR of target mRNAs (By similarity). Promotes formation of some phase-separated membraneless compartment by undergoing liquid-liquid phase separation upon binding to AREs-containing mRNAs : mRNAs storage into membraneless compartments regulates their translation and/or stability (By similarity). Acts as a regulator of adult hippocampal neurogenesis by regulating translation and/or stability of NOG mRNA, thereby preventing NOG protein expression in the dentate gyrus (By similarity).

See full target information FXR2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:1008 PubMed36446779

2022

mA-modified circFNDC3B inhibits colorectal cancer stemness and metastasis via RNF41-dependent ASB6 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zeng,Jin-Feng Zhu,Jian Guo,Gen-Jie Huang,Li-Sha Ai,Yu Zeng,Wang-Jun Liao

Analytical cellular pathology (Amsterdam) 2022:8275574 PubMed36340269

2022

Transcription Factor FXR Activates DHRS9 to Inhibit the Cell Oxidative Phosphorylation and Suppress Colon Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlai Zhao,Yigang Wang,Yang Wang,Jianchao Gao,Haichao Yang,Xiaotang Wu,Hua Li

Cell 185:2035-2056.e33 PubMed35688132

2022

The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Erinc Hallacli,Can Kayatekin,Sumaiya Nazeen,Xiou H Wang,Zoe Sheinkopf,Shubhangi Sathyakumar,Souvarish Sarkar,Xin Jiang,Xianjun Dong,Roberto Di Maio,Wen Wang,Matthew T Keeney,Daniel Felsky,Jackson Sandoe,Aazam Vahdatshoar,Namrata D Udeshi,D R Mani,Steven A Carr,Susan Lindquist,Philip L De Jager,David P Bartel,Chad L Myers,J Timothy Greenamyre,Mel B Feany,Shamil R Sunyaev,Chee Yeun Chung,Vikram Khurana

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FXR2 antibody

Immunoprecipitation - Anti-FXR2 antibody (AB168852)

Western blot - Anti-FXR2 antibody (AB168852)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise