AB76597

Anti-FXYD1/PLM antibody

(5 Reviews)

(15 Publications)

Rabbit Polyclonal FXYD1/PLM antibody. Suitable for WB and reacts with Human samples. Cited in 15 publications.

View Alternative Names

PLM, FXYD1, Phospholemman, FXYD domain-containing ion transport regulator 1, Sodium/potassium-transporting ATPase subunit FXYD1

1 Images

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Western blot - Anti-FXYD1/PLM antibody (AB76597)

All lanes:

Western blot - Anti-FXYD1/PLM antibody (ab76597) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Human brain normal tissue lysate - membrane extract (<a href='/en-us/products/unavailable/human-brain-normal-tissue-lysate-membrane-extract-ab29456'>ab29456</a>) at 10 µg

Lane 3:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 4:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

true

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FXYD1 also known as phospholemman (PLM) is a small transmembrane protein with a molecular mass of approximately 15 kDa. It is expressed mainly in cardiac and skeletal muscles where it plays a mechanical role in regulating ion transport across cell membranes. This protein modulates the activity of the Na+/K+-ATPase pump influencing the transport of sodium and potassium ions which is important for maintaining cellular homeostasis.

Biological function summary

Phospholemman participates in the regulation of cardiac contractility and is involved in muscle relaxation processes. As a component of the Na+/K+-ATPase pump complex it affects the pump's affinity for sodium ions which is important for its normal function. The effector actions of FXYD1 are modulated by phosphorylation allowing it to serve as an important regulator of cardiac output under varying physiological conditions.

Pathways

Phospholemman features in the ion homeostasis and excitability pathway where its action on the Na+/K+-ATPase pump links it to regulation of cardiac action potential. This regulation involves interaction with other membrane proteins like ankyrin which further connects FXYD1 to integrative cardiovascular mechanisms. Additionally FXYD1 impacts membrane hyperpolarization positioning it in the pathway that controls cell excitability and signal transduction in muscle tissues.

Dysregulation of FXYD1 is linked to heart failure and arrhythmias. Altered expression or function of this protein can affect Na+/K+-ATPase efficiency leading to imbalanced ion transport and compromised cardiac function. Interaction with proteins such as cAMP-dependent protein kinase also implicates FXYD1 in various cardiac pathologies influencing the therapeutic targeting of this protein in cardiovascular diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell. Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated. Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone.

See full target information FXYD1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0299665 PubMed38512906

2024

Targeted degradation of zDHHC-PATs decreases substrate S-palmitoylation.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjie Bai,Emily Gallen,Sarah Memarzadeh,Jacqueline Howie,Xing Gao,Chien-Wen S Kuo,Elaine Brown,Simon Swingler,Sam J Wilson,Michael J Shattock,David J France,William Fuller

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23535 PubMed38466300

2024

Cysteine post-translational modifications regulate protein interactions of caveolin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona Ashford,Chien-Wen Kuo,Emma Dunning,Elaine Brown,Sarah Calagan,Izzy Jayasinghe,Colin Henderson,William Fuller,Krzysztof Wypijewski

Cell reports 43:113679 PubMed38236777

2024

Glutathione-dependent depalmitoylation of phospholemman by peroxiredoxin 6.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline Howie,Lindsay B Tulloch,Elaine Brown,Louise Reilly,Fiona B Ashford,Jennifer Kennedy,Krzysztof J Wypijewski,Karen L Aughton,Jason K C Mak,Michael J Shattock,Niall J Fraser,William Fuller

Frontiers in physiology 13:1023237 PubMed36277202

2022

Dynamic but discordant alterations in zDHHC5 expression and palmitoylation of its substrates in cardiac pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Main,Andri Boguslavskyi,Jacqueline Howie,Chien-Wen Kuo,Aileen Rankin,Francis L Burton,Godfrey L Smith,Roger Hajjar,George S Baillie,Kenneth S Campbell,Michael J Shattock,William Fuller

American journal of physiology. Heart and circulatory physiology 321:H650-H662 PubMed34448639

2021

The α2-isoform of the Na/K-ATPase protects against pathological remodeling and β-adrenergic desensitization after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Cellini,Dorina Höfler,Paula A Arias-Loza,Sandra Bandleon,Tanja Langsenlehner,Michael Kohlhaas,Christoph Maack,Wolfgang R Bauer,Petra Eder-Negrin

Hypertension (Dallas, Tex. : 1979) 77:2104-2116 PubMed33934624

2021

FXYD1 Is Protective Against Vascular Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen J Bubb,Owen Tang,Carmine Gentile,Seyed M Moosavi,Thomas Hansen,Chia-Chi Liu,Belinda A Di Bartolo,Gemma A Figtree

The Journal of general physiology 152: PubMed33231612

2020

FXYD protein isoforms differentially modulate human Na/K pump function.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan J Meyer,Sharan Bijlani,Marilina de Sautu,Kerri Spontarelli,Victoria C Young,Craig Gatto,Pablo Artigas

Communications biology 3:411 PubMed32737405

2020

Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona Plain,Jacqueline Howie,Jennifer Kennedy,Elaine Brown,Michael J Shattock,Niall J Fraser,William Fuller

Cell research 30:1109-1126 PubMed32690901

2020

Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Li Yang,Yan-Chun Wang,Zi-Ran Xu,Ye Feng,Jing Zhang,Yi Wang,Cheng-Ran Xu

The Journal of physiology 598:1339-1359 PubMed30811606

2019

Changes in cellular Ca and Na regulation during the progression towards heart failure in the guinea pig.

Applications

Unspecified application

Species

Unspecified reactive species

H-Y Ke,H-Y Yang,A J Francis,T P Collins,H Surendran,A Alvarez-Laviada,J M Firth,K T MacLeod

View all publications

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Product promise

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Anti-FXYD1/PLM antibody

Western blot - Anti-FXYD1/PLM antibody (AB76597)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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