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AB251062

Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free

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Rabbit Recombinant Monoclonal FXYD1/PLM antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

View Alternative Names

PLM, FXYD1, Phospholemman, FXYD domain-containing ion transport regulator 1, Sodium/potassium-transporting ATPase subunit FXYD1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (AB251062)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (AB251062)

This data was developed using ab191397, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FXYD1/PLM with ab191397 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset : Negative control : using PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (AB251062)
  • IP

Supplier Data

Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (AB251062)

This data was developed using ab191397, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human fetal livert lysate immunoprecipitated using ab191397 at 1/90 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16081] (<a href='/en-us/products/primary-antibodies/fxyd1-plm-antibody-epr16081-ab191397'>ab191397</a>)

Predicted band size: 10 kDa

false

Western blot - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (AB251062)
  • WB

Supplier Data

Western blot - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (AB251062)

This data was developed using ab191397, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FXYD1/PLM antibody [EPR16081] (<a href='/en-us/products/primary-antibodies/fxyd1-plm-antibody-epr16081-ab191397'>ab191397</a>) at 1/10000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Human fetal heart tissue lysate at 20 µg

Lane 3:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16081

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab251062 is the carrier-free version of ab191397.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FXYD1 also known as phospholemman (PLM) is a small transmembrane protein with a molecular mass of approximately 15 kDa. It is expressed mainly in cardiac and skeletal muscles where it plays a mechanical role in regulating ion transport across cell membranes. This protein modulates the activity of the Na+/K+-ATPase pump influencing the transport of sodium and potassium ions which is important for maintaining cellular homeostasis.
Biological function summary

Phospholemman participates in the regulation of cardiac contractility and is involved in muscle relaxation processes. As a component of the Na+/K+-ATPase pump complex it affects the pump's affinity for sodium ions which is important for its normal function. The effector actions of FXYD1 are modulated by phosphorylation allowing it to serve as an important regulator of cardiac output under varying physiological conditions.

Pathways

Phospholemman features in the ion homeostasis and excitability pathway where its action on the Na+/K+-ATPase pump links it to regulation of cardiac action potential. This regulation involves interaction with other membrane proteins like ankyrin which further connects FXYD1 to integrative cardiovascular mechanisms. Additionally FXYD1 impacts membrane hyperpolarization positioning it in the pathway that controls cell excitability and signal transduction in muscle tissues.

Dysregulation of FXYD1 is linked to heart failure and arrhythmias. Altered expression or function of this protein can affect Na+/K+-ATPase efficiency leading to imbalanced ion transport and compromised cardiac function. Interaction with proteins such as cAMP-dependent protein kinase also implicates FXYD1 in various cardiac pathologies influencing the therapeutic targeting of this protein in cardiovascular diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell. Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated. Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone.
See full target information FXYD1
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Product promise

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