AB251304

Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal FXYD1/PLM antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

PLM, FXYD1, Phospholemman, FXYD domain-containing ion transport regulator 1, Sodium/potassium-transporting ATPase subunit FXYD1

4 Images

Supplier Data

Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

This data was developed using ab200204, the same antibody clone in a different buffer formulation.

FXYD1/PLM was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal heart whole cell lysate 10ug (Input).

Lane 2 : ab200204 IP in Human fetal heart whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] (<a href='/en-us/products/primary-antibodies/fxyd1-plm-antibody-epr16082-ab200204'>ab200204</a>)

Predicted band size: 10 kDa

Observed band size: 12 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

This data was developed using ab200204, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FXYD1/PLM antibody [EPR16082] (<a href='/en-us/products/primary-antibodies/fxyd1-plm-antibody-epr16082-ab200204'>ab200204</a>) at 1/10000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 10 kDa

Observed band size: 12 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

This data was developed using ab200204, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FXYD1/PLM antibody [EPR16082] (<a href='/en-us/products/primary-antibodies/fxyd1-plm-antibody-epr16082-ab200204'>ab200204</a>) at 1/1000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 10 kDa

Observed band size: 12 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

This data was developed using ab200204, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FXYD1/PLM antibody [EPR16082] (<a href='/en-us/products/primary-antibodies/fxyd1-plm-antibody-epr16082-ab200204'>ab200204</a>) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 10 kDa

Observed band size: 12 kDa

false

Exposure time: 3min

Unconjugated

Anti-FXYD1/PLM antibody [EPR16082]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16082

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251304 is the carrier-free version of ab200204.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FXYD1 also known as phospholemman (PLM) is a small transmembrane protein with a molecular mass of approximately 15 kDa. It is expressed mainly in cardiac and skeletal muscles where it plays a mechanical role in regulating ion transport across cell membranes. This protein modulates the activity of the Na+/K+-ATPase pump influencing the transport of sodium and potassium ions which is important for maintaining cellular homeostasis.

Biological function summary

Phospholemman participates in the regulation of cardiac contractility and is involved in muscle relaxation processes. As a component of the Na+/K+-ATPase pump complex it affects the pump's affinity for sodium ions which is important for its normal function. The effector actions of FXYD1 are modulated by phosphorylation allowing it to serve as an important regulator of cardiac output under varying physiological conditions.

Pathways

Phospholemman features in the ion homeostasis and excitability pathway where its action on the Na+/K+-ATPase pump links it to regulation of cardiac action potential. This regulation involves interaction with other membrane proteins like ankyrin which further connects FXYD1 to integrative cardiovascular mechanisms. Additionally FXYD1 impacts membrane hyperpolarization positioning it in the pathway that controls cell excitability and signal transduction in muscle tissues.

Dysregulation of FXYD1 is linked to heart failure and arrhythmias. Altered expression or function of this protein can affect Na+/K+-ATPase efficiency leading to imbalanced ion transport and compromised cardiac function. Interaction with proteins such as cAMP-dependent protein kinase also implicates FXYD1 in various cardiac pathologies influencing the therapeutic targeting of this protein in cardiovascular diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell. Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated. Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone.

See full target information FXYD1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free

Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (AB251304)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise