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AB251433

Anti-FXYD3 antibody [EPR17160] - BSA and Azide free

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Rabbit Recombinant Monoclonal FXYD3 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

View Alternative Names

MAT8, PLML, FXYD3, FXYD domain-containing ion transport regulator 3, Chloride conductance inducer protein Mat-8, Mammary tumor 8 kDa protein, Phospholemman-like, Sodium/potassium-transporting ATPase subunit FXYD3

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)

This data was developed using ab205534, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling FXYD3 with ab205534 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab205534 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)

This data was developed using ab205534, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling FXYD3 with ab205534 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane and cytoplasmic staining on human breast carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)

This data was developed using ab205534, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling FXYD3 with ab205534 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab205534 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)

This data was developed using ab205534, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling FXYD3 with ab205534 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

Western blot - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)
  • WB

Supplier Data

Western blot - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)

This data was developed using ab205534, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FXYD3 antibody [EPR17160] (<a href='/en-us/products/primary-antibodies/fxyd3-antibody-epr17160-ab205534'>ab205534</a>) at 1/1000 dilution

All lanes:

Human stomach lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 9 kDa

Observed band size: 12 kDa

false

Exposure time: 3min

Western blot - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)
  • WB

Supplier Data

Western blot - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)

This data was developed using ab205534, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FXYD3 antibody [EPR17160] (<a href='/en-us/products/primary-antibodies/fxyd3-antibody-epr17160-ab205534'>ab205534</a>) at 1/5000 dilution

All lanes:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 9 kDa

Observed band size: 12 kDa

false

Exposure time: 30s

Western blot - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)
  • WB

Supplier Data

Western blot - Anti-FXYD3 antibody [EPR17160] - BSA and Azide free (AB251433)

This data was developed using ab205534, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FXYD3 antibody [EPR17160] (<a href='/en-us/products/primary-antibodies/fxyd3-antibody-epr17160-ab205534'>ab205534</a>) at 1/1000 dilution

All lanes:

Human colon cancer lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 9 kDa

Observed band size: 12 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17160

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab251433 is the carrier-free version of ab205534.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FXYD3 also known as Mat-8 plays a role as a regulatory subunit associated with the Na+/K+-ATPase pump modulating its ion transport activity. The protein has a molecular weight of approximately 10 kDa. FXYD3 is expressed in various tissues but it shows higher levels in epithelial tissues particularly in gastrointestinal and mammary tissues. The protein consists of a single transmembrane domain which suggests its function in modulating ion exchange across membranes.
Biological function summary

FXYD3 modulates ion transport processes important for maintaining cellular homeostasis. It forms part of the larger Na+/K+-ATPase complex interacting with other subunits to influence the pump's activity. This association affects the electrochemical gradients across the cell membrane impacting various cellular processes including volume regulation pH balance and nutrient uptake. Therefore FXYD3 is involved in key physiological roles in tissues where electrolyte balance is essential.

Pathways

FXYD3 integrates into ion transport and signaling pathways including the sodium ion transmembrane transport pathway. It interacts closely with proteins like the α and β subunits of the Na+/K+-ATPase in this context regulating their pump activity. This integration with the sodium ion transmembrane transport and signal transduction pathways exemplifies FXYD3’s role in cellular ion homeostasis and how it may influence specific physiological responses.

FXYD3 shows links to cancer particularly breast cancer. Altered expression of FXYD3 has been observed in malignant tissues suggesting a contribution to tumor development and progression. The relationship between FXYD3 and proteins like E-cadherin in this context may indicate its role in cellular adhesion and migration alterations seen in cancer. Another disorder linked to FXYD3 is cystic fibrosis where its modulation of ion channels could affect disease severity or progression especially regarding electrolyte transport abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell (PubMed : 17077088). Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1 (PubMed : 21454534). Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes (PubMed : 7836447).. Isoform 1. Decreases the apparent K+ and Na+ affinity of the sodium/potassium-transporting ATPase over a large range of membrane potentials.. Isoform 2. Decreases the apparent K+ affinity of the sodium/potassium-transporting ATPase only at slightly negative and positive membrane potentials and increases the apparent Na+ affinity over a large range of membrane potentials.
See full target information FXYD3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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