AB181254

Anti-FXYD6/Plp antibody [EPR9004(2)]

Name: Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) | Abcam
Brand: Abcam Limited
SKU: AB181254
Price: 500 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal FXYD6/Plp antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

UNQ521/PRO1056, FXYD6, FXYD domain-containing ion transport regulator 6, Phosphohippolin

3 Images

Lab

Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] (AB181254)

Purified ab250467 at 1 : 20 dilution (0.8μg) immunoprecipitating FXYD6/Plp in Human testis lysate.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254)

Lane 1:

Human testis lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/fxyd6-plp-antibody-epr90042-bsa-and-azide-free-ab250467'>ab250467</a> + Human testis lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab181254 in human testis lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 11 kDa

false

Unknown

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (AB181254)

All lanes:

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/10000 dilution

All lanes:

Human testis lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

false

Unknown

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (AB181254)

All lanes:

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

false

Carrier free

Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9004(2)

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FXYD6 also known as phospholemman-like protein (Plp) is a member of the FXYD family that regulates ion transport. This protein has a mass of around 9 kDa and demonstrates specificity in modulating sodium-potassium (Na+/K+) ATPase activity. The expression of FXYD6 is quite widespread but appears higher in tissues such as brain and kidney suggesting its important roles in these organs' physiological functions. It influences ion balance and potentially affects cellular excitability and fluid transport through its modulation of ion channels and pumps.

Biological function summary

FXYD6 impacts multiple cellular mechanisms mainly through its association with Na+/K+ ATPase complex. It influences ion gradients across the plasma membrane an essential factor in maintaining cell volume and resting membrane potential. Interactions within this complex suggest FXYD6 plays an important part in fine-tuning ion pump efficiency affecting the overall cellular ionic homeostasis. Its precise role within the ATPase complex is important for adapting the function of these pumps to specific physiological conditions.

Pathways

FXYD6 integrates into ion transport and signal transduction pathways particularly those involving Na+/K+ ATPase. It cooperates with other FXYD proteins like FXYD2 in adjusting ion pump functionality ensuring cells respond adequately to physiological stimuli. FXYD6 modulates the ATPase in neural and renal tissues linking it to neural signaling pathways such as neurotransmission processes and kidney regulation pathways.

FXYD6 has associations with schizophrenia and renal diseases. In schizophrenia alterations in FXYD6 expression or function can affect neural ion balance potentially contributing to the disorder's pathophysiology. In renal diseases aberrant FXYD6 activity can disrupt kidney ion transport implicating it in conditions like hypertension. Through these disorders FXYD6 connects with proteins such as FXYD2 and gamma subunits of Na+/K+ ATPase impacting the pathological outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. Reduces the apparent affinity for intracellular Na(+) with no change in the apparent affinity for extracellular K(+) (PubMed : 33231612). In addition to modulating NKA kinetics, may also function as a regulator of NKA localization to the plasma membrane (By similarity).

See full target information FXYD6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 12:35 PubMed38414005

2024

Decoding of the surfaceome and endocytome in primary glioblastoma cells identifies potential target antigens in the hypoxic tumor niche.

Applications

Unspecified application

Species

Unspecified reactive species

Kelin Gonçalves de Oliveira,Anna Bång-Rudenstam,Sarah Beyer,Axel Boukredine,Hugo Talbot,Valeria Governa,Maria C Johansson,Ann-Sofie Månsson,Karin Forsberg-Nilsson,Johan Bengzon,Johan Malmström,Charlotte Welinder,Mattias Belting

Nutrients 16: PubMed38201894

2024

BMP8B Activates Both SMAD2/3 and NF-κB Signals to Inhibit the Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shenjie Zhong,Xueqing Du,Jing Gao,Guangdong Ji,Zhenhui Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FXYD6/Plp antibody [EPR9004(2)]

Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] (AB181254)

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (AB181254)

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (AB181254)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise