Rabbit Recombinant Monoclonal FXYD6/Plp antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IP | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Rat | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. Reduces the apparent affinity for intracellular Na(+) with no change in the apparent affinity for extracellular K(+) (PubMed:33231612). In addition to modulating NKA kinetics, may also function as a regulator of NKA localization to the plasma membrane (By similarity).
UNQ521/PRO1056, UNQ521/PRO1056, FXYD6, FXYD domain-containing ion transport regulator 6, Phosphohippolin
Rabbit Recombinant Monoclonal FXYD6/Plp antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR9004(2)
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab250467 is the carrier-free version of Anti-FXYD6/Plp antibody [EPR9004(2)] ab181254.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
FXYD6 also known as phospholemman-like protein (Plp) is a member of the FXYD family that regulates ion transport. This protein has a mass of around 9 kDa and demonstrates specificity in modulating sodium-potassium (Na+/K+) ATPase activity. The expression of FXYD6 is quite widespread but appears higher in tissues such as brain and kidney suggesting its important roles in these organs' physiological functions. It influences ion balance and potentially affects cellular excitability and fluid transport through its modulation of ion channels and pumps.
FXYD6 impacts multiple cellular mechanisms mainly through its association with Na+/K+ ATPase complex. It influences ion gradients across the plasma membrane an essential factor in maintaining cell volume and resting membrane potential. Interactions within this complex suggest FXYD6 plays an important part in fine-tuning ion pump efficiency affecting the overall cellular ionic homeostasis. Its precise role within the ATPase complex is important for adapting the function of these pumps to specific physiological conditions.
FXYD6 integrates into ion transport and signal transduction pathways particularly those involving Na+/K+ ATPase. It cooperates with other FXYD proteins like FXYD2 in adjusting ion pump functionality ensuring cells respond adequately to physiological stimuli. FXYD6 modulates the ATPase in neural and renal tissues linking it to neural signaling pathways such as neurotransmission processes and kidney regulation pathways.
FXYD6 has associations with schizophrenia and renal diseases. In schizophrenia alterations in FXYD6 expression or function can affect neural ion balance potentially contributing to the disorder's pathophysiology. In renal diseases aberrant FXYD6 activity can disrupt kidney ion transport implicating it in conditions like hypertension. Through these disorders FXYD6 connects with proteins such as FXYD2 and gamma subunits of Na+/K+ ATPase impacting the pathological outcomes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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All lanes: Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (Anti-FXYD6/Plp antibody [EPR9004(2)] ab181254) at 1/10000 dilution
All lanes: Human testis lysate
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
This data was developed using ab250467, the same antibody clone in a different buffer formulation.
Purified ab250467 at 1:20 dilution (0.8μg) immunoprecipitating FXYD6/Plp in Human testis lysate.
Lane 1 (input): Human testis lysate 10 ?g
Lane 2 (+): ab250467 + Human testis lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FXYD6/Plp antibody [EPR9004(2)] ab181254 in human testis lysate.
VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] (Anti-FXYD6/Plp antibody [EPR9004(2)] ab181254)
Predicted band size: 11 kDa
All lanes: Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (Anti-FXYD6/Plp antibody [EPR9004(2)] ab181254) at 1/1000 dilution
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
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