AB250467

Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal FXYD6/Plp antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

UNQ521/PRO1056, FXYD6, FXYD domain-containing ion transport regulator 6, Phosphohippolin

3 Images

Lab

Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (AB250467)

This data was developed using ab250467, the same antibody clone in a different buffer formulation.
Purified ab250467 at 1 : 20 dilution (0.8μg) immunoprecipitating FXYD6/Plp in Human testis lysate.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] (<a href='/en-us/products/primary-antibodies/fxyd6-plp-antibody-epr90042-ab181254'>ab181254</a>)

Lane 1:

Human testis lysate at 10 µg

Lane 2:

ab250467 + Human testis lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/fxyd6-plp-antibody-epr90042-ab181254'>ab181254</a> in human testis lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 11 kDa

false

Unknown

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (AB250467)

All lanes:

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (<a href='/en-us/products/primary-antibodies/fxyd6-plp-antibody-epr90042-ab181254'>ab181254</a>) at 1/10000 dilution

All lanes:

Human testis lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

false

Unknown

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (AB250467)

All lanes:

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] (<a href='/en-us/products/primary-antibodies/fxyd6-plp-antibody-epr90042-ab181254'>ab181254</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

false

Unconjugated

Anti-FXYD6/Plp antibody [EPR9004(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9004(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250467 is the carrier-free version of ab181254.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FXYD6 also known as phospholemman-like protein (Plp) is a member of the FXYD family that regulates ion transport. This protein has a mass of around 9 kDa and demonstrates specificity in modulating sodium-potassium (Na+/K+) ATPase activity. The expression of FXYD6 is quite widespread but appears higher in tissues such as brain and kidney suggesting its important roles in these organs' physiological functions. It influences ion balance and potentially affects cellular excitability and fluid transport through its modulation of ion channels and pumps.

Biological function summary

FXYD6 impacts multiple cellular mechanisms mainly through its association with Na+/K+ ATPase complex. It influences ion gradients across the plasma membrane an essential factor in maintaining cell volume and resting membrane potential. Interactions within this complex suggest FXYD6 plays an important part in fine-tuning ion pump efficiency affecting the overall cellular ionic homeostasis. Its precise role within the ATPase complex is important for adapting the function of these pumps to specific physiological conditions.

Pathways

FXYD6 integrates into ion transport and signal transduction pathways particularly those involving Na+/K+ ATPase. It cooperates with other FXYD proteins like FXYD2 in adjusting ion pump functionality ensuring cells respond adequately to physiological stimuli. FXYD6 modulates the ATPase in neural and renal tissues linking it to neural signaling pathways such as neurotransmission processes and kidney regulation pathways.

FXYD6 has associations with schizophrenia and renal diseases. In schizophrenia alterations in FXYD6 expression or function can affect neural ion balance potentially contributing to the disorder's pathophysiology. In renal diseases aberrant FXYD6 activity can disrupt kidney ion transport implicating it in conditions like hypertension. Through these disorders FXYD6 connects with proteins such as FXYD2 and gamma subunits of Na+/K+ ATPase impacting the pathological outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. Reduces the apparent affinity for intracellular Na(+) with no change in the apparent affinity for extracellular K(+) (PubMed : 33231612). In addition to modulating NKA kinetics, may also function as a regulator of NKA localization to the plasma membrane (By similarity).

See full target information FXYD6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free

Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (AB250467)

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (AB250467)

Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (AB250467)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise