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AB247426

Anti-FYB antibody [EPR2547Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal FYB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

FYB, SLAP130, FYB1, FYN-binding protein 1, Adhesion and degranulation promoting adaptor protein, FYB-120/130, FYN-T-binding protein, SLAP-130, SLP-76-associated phosphoprotein, ADAP, p120/p130

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EPR2547Y] - BSA and Azide free (AB247426)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EPR2547Y] - BSA and Azide free (AB247426)

This data was developed using ab76006, the same antibody clone in a different buffer formulation.

ab76006, at 1/100 dilution, staining FYB in human tonsil by immunohistochemistry using paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-FYB antibody [EPR2547Y] - BSA and Azide free (AB247426)
  • WB

Unknown

Western blot - Anti-FYB antibody [EPR2547Y] - BSA and Azide free (AB247426)

This data was developed using ab76006, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FYB antibody [EPR2547Y] (<a href='/en-us/products/primary-antibodies/fyb-antibody-epr2547y-ab76006'>ab76006</a>) at 1/500 dilution

All lanes:

TF-1 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 85 kDa

Observed band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2547Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247426 is the carrier-free version of ab76006.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein 'FYB' also known as Fyn-binding protein or ADAP (Adhesion and Degranulation-promoting Adapter Protein) is a cytosolic adaptor protein with a molecular mass of approximately 85 kDa. This protein is important for intracellular signaling pathways as it acts as a link between various surface receptors and downstream signaling molecules. FYB is prominently expressed in hematopoietic cells with its presence noted in T-cells where it facilitates effective immune responses. This makes anti-FYB antibodies valuable tools for studying T-cell signaling and immune regulation.
Biological function summary

The Fyn-binding protein plays significant roles in modulating immune responses by interacting with the cytoskeleton and various signaling components. FYB does not normally function alone but exists as part of the LAT (Linker for Activation of T-cells) signalosome complex a multi-protein structure essential for T-cell receptor (TCR) signaling. It influences cytoskeletal rearrangement cell adhesion and migration impacting the immune cell's ability to reach sites of inflammation or infection. Its interaction with integrins further enhances these cellular processes.

Pathways

FYB is integral to T-cell receptor signaling and integrin-mediated signal transduction pathways. In TCR signaling FYB works alongside core proteins such as SLP-76 and Vav1 to propagate activation signals necessary for T-cell activation and proliferation. Within integrin signaling FYB collaborates with Talin and Kindlin to regulate cell adhesion and motility fundamental for immune surveillance and response tasks. The tight regulation of these pathways ensures that immune cells can properly adapt to various immune challenges.

Irregular FYB function associates with defective immune responses and autoimmune conditions such as systemic lupus erythematosus and rheumatoid arthritis. These conditions arise from aberrant activation or suppression of T-cell signaling where FYB as part of the LAT signalosome indirectly contributes to the pathophysiology. Furthermore FYB interactions with proteins like SLP-76 and Vav1 highlight its potential role in these diseases making it a target for research into therapeutic interventions for T-cell mediated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells (By similarity). May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton (PubMed : 10747096, PubMed : 16980616). Modulates the expression of IL2 (By similarity). Involved in platelet activation (By similarity). Prevents the degradation of SKAP1 and SKAP2 (PubMed : 15849195). May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells (By similarity).
See full target information FYB1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com