Anti-FYCO1 antibody [EPR28360-524]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling FYCO1 with ab324455 at 1/200 (2.55 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human cervical carcinoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling FYCO1 with ab324455 at 1/200 (2.55 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human colon carcinoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling FYCO1 with ab324455 at 1/200 (2.55 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human skeletal muscle.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling FYCO1 with ab324455 at 1/200 (2.55 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression tissue : Nearly no staining on human cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling FYCO1 with ab324455 at 1/200 (2.55 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human mammary gland.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band are likely to be degradation products.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FYCO1 antibody [EPR28360-524] (ab324455) at 1/1000 dilution

Lane 1:

HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting FYCO1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, cerebellum (PMID : 34215815)

The bands beneath the target band are likely to be degradation products.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FYCO1 antibody [EPR28360-524] (ab324455) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-FYCO1 antibody [EPR28360-524] (AB324455)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : SK-N-BE(2), THP-1.

In lanes 1-7, the lysate was stored at -80°C prior to Western Blotting. The bands beneath the target band (200 kDa) are likey to be degradation products. In lanes 8-9, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FYCO1 antibody [EPR28360-524] (ab324455) at 1/1000 dilution

Lane 1:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate (Frozen lysates) at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (Frozen lysates) at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate (Frozen lysates) at 20 µg

Lane 4:

SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate (Frozen lysates) at 20 µg

Lane 5:

SK-MEL-28 (human malignant melanoma cell) whole cell lysate (Frozen lysates) at 20 µg

Lane 6:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate (Frozen lysates) at 20 µg

Lane 7:

THP-1 (human monocytic leukemia monocyte) whole cell lysate (Frozen lysates) at 20 µg

Lane 8:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (Fresh lysates) at 20 µg

Lane 9:

293T (human embryonic kidney epithelial cell) whole cell lysate (Fresh lysates) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 15s