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Rabbit Recombinant Monoclonal FYN antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 20 publications.


Images

Western blot - Anti-Fyn antibody [EPR5500] (AB125016), expandable thumbnail
  • Western blot - Anti-Fyn antibody [EPR5500] (AB125016), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] (AB125016), expandable thumbnail
  • Western blot - Anti-Fyn antibody [EPR5500] (AB125016), expandable thumbnail
  • Western blot - Anti-Fyn antibody [EPR5500] (AB125016), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Expected
Mouse
Tested
Not recommended
Tested
Rat
Tested
Not recommended
Expected

Tested
Tested

Species

Mouse

Dilution info
1/1000
Notes

-

Species

Rat

Dilution info
1/1000
Notes

-

Species

Human

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/500
Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance (PubMed:11536198, PubMed:15489916, PubMed:15557120, PubMed:16387660, PubMed:20100835, PubMed:7568038, PubMed:7822789). Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain (PubMed:15489916). Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions (PubMed:15489916). Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin) (PubMed:17194753). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT (PubMed:14707117, PubMed:15536091). Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage (PubMed:16841086). Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6 (PubMed:14761972, PubMed:18258597, PubMed:19179337). Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein (PubMed:11162638, PubMed:12788081, PubMed:19652227). Involved in reelin signaling by mediating phosphorylation of DAB1 following reelin (RELN)-binding to its receptor (By similarity). Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation (PubMed:22080863). Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation (PubMed:20028775). Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts (PubMed:18056706). CSK maintains LCK and FYN in an inactive form (By similarity). Promotes CD28-induced phosphorylation of VAV1 (PubMed:11005864). In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity). Can also promote CD244-mediated NK cell activation (PubMed:15713798).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal FYN antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 20 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR5500

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Fyn antibody [EPR5500] (ab125016), expandable thumbnail

    Western blot - Anti-Fyn antibody [EPR5500] (ab125016)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Fyn antibody [EPR5500] (ab125016) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 20 µg

    Lane 2: Ramos (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

    Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Fyn antibody [EPR5500] (ab125016), expandable thumbnail

    Western blot - Anti-Fyn antibody [EPR5500] (ab125016)

    False colour image of Western blot: Anti-Fyn antibody [EPR5500] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab125016 was shown to bind specifically to Fyn. A band was observed at 60 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in FYN knockout cell line Human FYN knockout HEK-293 cell line ab269630 (knockout cell lysate Human FYN knockout HEK-293 cell lysate ab272440). To generate this image, wild-type and FYN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Fyn antibody [EPR5500] (ab125016) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: FYN knockout HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] (ab125016), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] (ab125016)

    Immunofluorescence staining of Neuro-2a (Mouse neuroblastoma cell line) cells with purified ab125016 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1 in 500. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.

    The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab125016 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

  • Western blot - Anti-Fyn antibody [EPR5500] (ab125016), expandable thumbnail

    Western blot - Anti-Fyn antibody [EPR5500] (ab125016)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Fyn antibody [EPR5500] (ab125016) at 1/5000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Fyn antibody [EPR5500] (ab125016), expandable thumbnail

    Western blot - Anti-Fyn antibody [EPR5500] (ab125016)

    All lanes: Western blot - Anti-Fyn antibody [EPR5500] (ab125016) at 1/1000 dilution

    Lane 1: Ramos (Human Burkitt's lymphoma cell line) lysate at 10 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa

    Observed band size: 59 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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