Anti-Fyn antibody [FYN-01]

• Flow Cyt

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Flow Cytometry - Anti-Fyn antibody [FYN-01] (AB1881)

Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• ICC

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Immunocytochemistry - Anti-Fyn antibody [FYN-01] (AB1881)

Immunocytochemistry analysis of Fyn in human HeLa cell line using ab1881 (green) at 5 μL. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

• ICC

Supplier Data

Immunocytochemistry - Anti-Fyn antibody [FYN-01] (AB1881)

Immunofluorescence analysis of human primary fibroblasts labelling Fyn with ab1881 at 5 μL. Actin cytoskeleton was stained with phalloidin (red) and cell nuclei were stained with DAPI (blue).

• ICC

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Immunocytochemistry - Anti-Fyn antibody [FYN-01] (AB1881)

Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using ab1881 (red) at 5 μL. Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

• WB

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Western blot - Anti-Fyn antibody [FYN-01] (AB1881)

All lanes:

Western blot - Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/mL

All lanes:

Western blot - Recombinant human Fyn protein (<a href='/en-us/products/proteins-peptides/recombinant-human-fyn-protein-ab84696'>ab84696</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 60 kDa

true

Exposure time: 10s

• WB

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Western blot - Anti-Fyn antibody [FYN-01] (AB1881)

All lanes:

Western blot - Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/mL

All lanes:

Western blot - Recombinant human Fyn protein (<a href='/en-us/products/proteins-peptides/recombinant-human-fyn-protein-ab84696'>ab84696</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 60 kDa

true

Exposure time: 10s