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Mouse Monoclonal FYN antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tyrosine-protein kinase Fyn aa 1-200.


Images

Immunocytochemistry - Anti-Fyn antibody [FYN-01] (AB1881), expandable thumbnail
  • Flow Cytometry - Anti-Fyn antibody [FYN-01] (AB1881), expandable thumbnail
  • Western blot - Anti-Fyn antibody [FYN-01] (AB1881), expandable thumbnail
  • Immunocytochemistry - Anti-Fyn antibody [FYN-01] (AB1881), expandable thumbnail
  • Immunocytochemistry - Anti-Fyn antibody [FYN-01] (AB1881), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human Tyrosine-protein kinase Fyn aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P06241

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWB
Human
Tested
Expected
Tested
Tested
Mouse
Tested
Expected
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info
5 µg/mL
Notes

-

Species

Human

Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info
1 µg for 106 Cells
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info
2 µg/mL
Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance (PubMed:11536198, PubMed:15489916, PubMed:15557120, PubMed:16387660, PubMed:20100835, PubMed:7568038, PubMed:7822789). Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain (PubMed:15489916). Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions (PubMed:15489916). Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin) (PubMed:17194753). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT (PubMed:14707117, PubMed:15536091). Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage (PubMed:16841086). Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6 (PubMed:14761972, PubMed:18258597, PubMed:19179337). Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein (PubMed:11162638, PubMed:12788081, PubMed:19652227). Involved in reelin signaling by mediating phosphorylation of DAB1 following reelin (RELN)-binding to its receptor (By similarity). Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation (PubMed:22080863). Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation (PubMed:20028775). Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts (PubMed:18056706). CSK maintains LCK and FYN in an inactive form (By similarity). Promotes CD28-induced phosphorylation of VAV1 (PubMed:11005864). In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity). Can also promote CD244-mediated NK cell activation (PubMed:15713798).

Alternative names

Recommended products

Mouse Monoclonal FYN antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tyrosine-protein kinase Fyn aa 1-200.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human Tyrosine-protein kinase Fyn aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P06241
Clone number

FYN-01

Purification technique

Affinity purification Protein A

Specificity

The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases.

Concentration
Loading...
Purification notes

Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Fyn is a Src family tyrosine kinase with a molecular weight of approximately 59 kDa. It is often referred to as Fyn kinase and plays a significant role in signal transduction processes. This protein is observed in various tissues with high expression in the brain T cells and platelets. Fyn kinase is known for its function in cell communication and regulates different cellular processes in these tissues.

Biological function summary

Fyn kinase influences cell growth differentiation and survival. It is a part of larger signaling complexes including those accountable for the development and function of the central nervous system. The protein's activity is important for neural plasticity and supports synaptic communication particularly within the brain. Fyn can interact with other proteins in these complexes to help direct cellular responses to external signals.

Pathways

Fyn kinase is central to several intracellular signaling pathways such as the T cell receptor (TCR) signaling pathway and the integrin signaling pathway. The TCR signaling pathway involves the regulation of immune responses while the integrin signaling pathway contributes to cell adhesion and migration. Other proteins like Lck and Yes interact with Fyn within these pathways adding another layer of regulation and response in various cell types.

Associated diseases and disorders

Fyn kinase has associations with Alzheimer's disease and certain types of cancer. In Alzheimer's dysregulation of Fyn activity contributes to synaptic dysfunction and neuronal damage. In cancer aberrant Fyn signaling can affect cellular proliferation and metastasis. Proteins such as Tau in Alzheimer's disease and Src in cancer relate to Fyn through their respective pathological processes.

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6 product images

  • Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881), expandable thumbnail

    Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)

    Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using ab1881 (red) at 5 μL. Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

  • Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881), expandable thumbnail

    Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881)

    Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-Fyn antibody [FYN-01] (ab1881), expandable thumbnail

    Western blot - Anti-Fyn antibody [FYN-01] (ab1881)

    All lanes: Western blot - Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/mL

    All lanes: Western blot - Recombinant human Fyn protein (Recombinant human Fyn protein ab84696) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Exposure time: 10s

  • Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881), expandable thumbnail

    Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)

    Immunofluorescence analysis of human primary fibroblasts labelling Fyn with ab1881 at 5 μL. Actin cytoskeleton was stained with phalloidin (red) and cell nuclei were stained with DAPI (blue).

  • Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881), expandable thumbnail

    Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)

    Immunocytochemistry analysis of Fyn in human HeLa cell line using ab1881 (green) at 5 μL. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

  • Western blot - Anti-Fyn antibody [FYN-01] (ab1881), expandable thumbnail

    Western blot - Anti-Fyn antibody [FYN-01] (ab1881)

    All lanes: Western blot - Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/mL

    All lanes: Western blot - Recombinant human Fyn protein (Recombinant human Fyn protein ab84696) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Exposure time: 10s

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Product protocols

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