AB238975

Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal FYN phospho Y530 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, p59-Fyn, SLK, FYN

3 Images

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Immunoprecipitation - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free (AB238975)

This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide
ab188319 (purified) at 1 : 30 dilution (2μg) immunoprecipitating Fyn (phospho Y530) + Yes (phospho Y537) in HeLa treated with 50nM Pervandate for 1h whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Pervandate for 1h whole cell lysate 10μg
Lane 2 (+) : ab188319 & HeLa treated with 50nM Pervandate for 1h whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab188319 in HeLa treated with 50nM Pervandate for 1h whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] (<a href='/en-us/products/primary-antibodies/fyn-phospho-y530-yes1-phospho-y537-antibody-epr13512-ab188319'>ab188319</a>)

Predicted band size: 61 kDa

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Supplier Data

Western blot - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free (AB238975)

This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide.

All lanes:

Western blot - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] (<a href='/en-us/products/primary-antibodies/fyn-phospho-y530-yes1-phospho-y537-antibody-epr13512-ab188319'>ab188319</a>) at 1/20000 dilution

Lane 1:

Lysate from HeLa cells treated with pervanadate at 10 µg

Lane 2:

Lysate from untreated HeLa cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 59 kDa

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Lab

Western blot - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free (AB238975)

This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide.

All lanes:

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates. Then the membrane was incubated with phosphatase at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 61 kDa

Observed band size: 59 kDa

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Unconjugated

Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13512

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab238975 is the carrier-free version of ab188319.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fyn and Yes1 are Src family kinases key players in signal transduction processes within cells. They share similarities in function and mechanism often grouped together due to their roles in cellular phosphorylation events. Fyn and Yes1 have a molecular mass of approximately 59 kDa and are ubiquitously expressed in many tissues including brain thymus and T lymphocytes. Fyn known also as p59fyn contributes significantly to the regulation of immune response and neuronal signaling by phosphorylation of different substrates.

Biological function summary

Fyn and Yes1 influence various cellular processes such as cell growth differentiation and survival. They form part of the larger signaling complexes that regulate cytoskeleton organization helping in maintaining cell structure and motility. When activated they communicate changes in the cellular environment by phosphorylating tyrosine residues on target proteins affecting downstream signaling pathways.

Pathways

Both Fyn and Yes1 play major roles in the mitogen-activated protein kinase (MAPK) pathway and the phosphoinositide 3-kinase (PI3K) pathway. These pathways are fundamental for transmitting extracellular signals to the cell nucleus triggering responses like cell proliferation or survival. Through these pathways Fyn and Yes1 interact with various proteins including phospholipase C-gamma (PLCγ) and SH2 domain-containing proteins. Their activation leads to further signaling cascades that impact cellular outcomes.

Aberrant Fyn and Yes1 activity have been linked to cancer and neurodegenerative disorders. Overexpression or mutation of these kinases can lead to uncontrolled cell proliferation contributing to breast cancer. In the context of neurodegenerative diseases Fyn interacts with the amyloid precursor protein implicated in Alzheimer's disease. Understanding these connections provides potential therapeutic targets for intervention in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance (PubMed : 11536198, PubMed : 15489916, PubMed : 15557120, PubMed : 16387660, PubMed : 20100835, PubMed : 7568038, PubMed : 7822789). Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain (PubMed : 15489916). Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions (PubMed : 15489916). Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin) (PubMed : 17194753). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT (PubMed : 14707117, PubMed : 15536091). Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage (PubMed : 16841086). Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6 (PubMed : 14761972, PubMed : 18258597, PubMed : 19179337). Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein (PubMed : 11162638, PubMed : 12788081, PubMed : 19652227). Involved in reelin signaling by mediating phosphorylation of DAB1 following reelin (RELN)-binding to its receptor (By similarity). Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation (PubMed : 22080863). Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation (PubMed : 20028775). Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts (PubMed : 18056706). CSK maintains LCK and FYN in an inactive form (By similarity). Promotes CD28-induced phosphorylation of VAV1 (PubMed : 11005864). In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity). Can also promote CD244-mediated NK cell activation (PubMed : 15713798).

See full target information Tyrosine-protein kinase Fyn phospho Y530

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1579843 PubMed40661777

2025

Analysis of the molecular subtypes and prognostic models of anoikis-related genes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Shen,Kang Hou,Jifeng Zhang,Xiaodong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free

Immunoprecipitation - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free (AB238975)

Western blot - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free (AB238975)

Western blot - Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free (AB238975)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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