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AB125082

Anti-FYTTD1 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal FYTTD1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FYTTD1 aa 1-50.

View Alternative Names

UIF, FYTTD1, UAP56-interacting factor, Forty-two-three domain-containing protein 1, Protein 40-2-3

2 Images
Immunoprecipitation - Anti-FYTTD1 antibody (AB125082)
  • IP

Unknown

Immunoprecipitation - Anti-FYTTD1 antibody (AB125082)

Detection of FYTTD1 by Western Blot of Immunprecipitate.
ab125082 at 1µg/ml staining FYTTD1 in 293T whole cell lysate immunoprecipitated using ab125082 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FYTTD1 antibody (ab125082)

Predicted band size: 36 kDa

false

Western blot - Anti-FYTTD1 antibody (AB125082)
  • WB

Unknown

Western blot - Anti-FYTTD1 antibody (AB125082)

All lanes:

Western blot - Anti-FYTTD1 antibody (ab125082) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

HeLa cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 36 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human FYTTD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96QD9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab125082 is affinity purified using an epitope specific to FYTTD1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FYTTD1 also known as UIF or U2AF65-interacting factor plays an essential role mechanically within the spliceosome. The protein has a molecular mass of approximately 63 kDa. FYTTD1 expresses widely across human tissues but shows higher expression in the nucleoplasm of cells. The protein facilitates interactions in pre-mRNA splicing by associating with other components of the spliceosomal complex which is critical for mRNA processing.
Biological function summary

FYTTD1 influences pre-mRNA splicing by forming part of the spliceosome complex. Research shows it interacts with U2AF65 a splicing factor necessary for promoting the assembly of the spliceosome complex on pre-mRNA. FYTTD1 ensures proper exon recognition and the removal of introns contributing to mRNA maturation. Its function is vital within the spliceosomal machinery maintaining the fidelity of gene expression.

Pathways

FYTTD1 supports efficient splicing and processing of pre-mRNA which directly affects mRNA transcript stability and protein synthesis pathways. It ties closely to the spliceosomal pathway and the gene expression regulation process. FYTTD1 works alongside proteins like U2AF65 and SF3B1 illustrating its participation in complex splicing mechanisms that regulate cellular metabolic activities and genome stability.

FYTTD1 is implicated in conditions such as cancer and neurodegenerative disorders. Abnormal splicing involving FYTTD1 can lead to aberrations in gene expression contributing to oncogenesis or faulty neuronal functioning. Studies link FYTTD1 dysregulation with changes in other proteins like SF3B1 and U2AF65 which exhibit altered activity in various cancers and neurological conditions highlighting the importance of its regulated expression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for mRNA export from the nucleus to the cytoplasm. Acts as an adapter that uses the DDX39B/UAP56-NFX1 pathway to ensure efficient mRNA export and delivering to the nuclear pore. Associates with spliced and unspliced mRNAs simultaneously with ALYREF/THOC4.
See full target information FYTTD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:27119 PubMed39511334

2024

Protein-RNA interaction dynamics reveal key regulators of oncogenic KRAS-driven cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ka-Yun Ban,Yong-Woo Na,Juhan Song,Jong-Seo Kim,Jimi Kim

PloS one 13:e0197266 PubMed29768513

2018

Increased mitochondrial fission is critical for hypoxia-induced pancreatic beta cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Da Zhang,Yanfang Liu,Yao Tang,Xiaofeng Wang,Zhichao Li,Rui Li,Zhenyu Ti,Weidong Gao,Jigang Bai,Yi Lv
View all publications
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