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AB254588

Anti-FZD10 antibody

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(1 Publication)

Rabbit Polyclonal FZD10 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FZD10 aa 450-500.

View Alternative Names

CD350, Frizzled-10, Fz-10, hFz10, FzE7, FZD10

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FZD10 antibody (AB254588)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FZD10 antibody (AB254588)

Paraffin-embedded human colon tissue stained for FZD10 using ab254588 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-FZD10 antibody (AB254588)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FZD10 antibody (AB254588)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling FZD10 using ab254588 at 4μg/ml (green) in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FZD10 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q9ULW2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Frizzled-10 (FZD10) also known as CD350 is a receptor in the frizzled family of proteins. It acts as a receptor for Wnt signaling proteins typically involved in communications between cells. The receptor belongs to the larger group of G-protein-coupled receptors and contains a seven-transmembrane domain structure. Its molecular mass is about 64 kDa. FZD10 is prominently expressed in various tissues including the brain and certain regions of the gastrointestinal tract with higher expression often seen in fetal tissues compared to adult tissues.
Biological function summary

FZD10 plays a significant role in the Wnt signaling pathway that affects cell development differentiation and proliferation. It usually forms part of a receptor complex that includes other proteins like LRP5/6 and Dishevelled. The interaction of FZD10 with Wnt ligands activates downstream signaling cascades important for embryonic development and maintaining stem cell states. Its involvement in these processes underlines its role in regulating key cellular dynamics.

Pathways

FZD10 is central to both the canonical Wnt/β-catenin pathway and non-canonical Wnt pathways. These pathways orchestrate critical cellular functions such as gene expression cytoskeletal arrangement and cellular movement. FZD10 collaborates closely with other frizzled receptors and Wnt proteins like Wnt3a to modulate these pathways. By regulating the stabilization and localization of β-catenin FZD10 influences various downstream target genes essential for cellular activities.

FZD10 shows association with certain cancers particularly synovial sarcoma and colon cancer. Misregulated expression of FZD10 contributes to oncogenic processes by abnormally activating Wnt signaling pathways which in turn affects cellular proliferation and survival. Additionally its association with Wnt signaling proteins such as β-catenin in these disease states emphasizes its impact on tumorigenesis and progression. Understanding FZD10's role offers potential therapeutic targets for interventions in cancers where this pathway is implicated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).
See full target information FZD10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed34771722

2021

VISTA Is a Diagnostic Biomarker and Immunotherapy Target of Aggressive Feline Mammary Carcinoma Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia Gameiro,Catarina Nascimento,Jorge Correia,Fernando Ferreira
View all publications
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