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AB325856

Anti-FZD9 antibody [HL1675] - BSA and Azide free

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Rabbit Recombinant Monoclonal Frizzled 9 antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FZD9.

View Alternative Names

CD349, FZD3, FZD9, Frizzled-9, Fz-9, hFz9, FzE6

3 Images
Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (AB325856)
  • WB

Supplier Data

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (AB325856)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 9 antibody ab325856 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (ab325856) at 1/5000 dilution

All lanes:

MG-83 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (AB325856)
  • WB

Supplier Data

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (AB325856)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325856 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (ab325856) at 1/5000 dilution

All lanes:

U87-MG whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (AB325856)
  • WB

Supplier Data

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (AB325856)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325856 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FZD9 antibody [HL1675] - BSA and Azide free (ab325856) at 1/1000 dilution

Lane 1:

Non-transfected (–) whole cell extracts at 30 µg

Lane 2:

FZD9 shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1675

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human FZD9.

O00144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for WNT2 that is coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (By similarity). Plays a role in neuromuscular junction (NMJ) assembly by negatively regulating the clustering of acetylcholine receptors (AChR) through the beta-catenin canonical signaling pathway (By similarity). May play a role in neural progenitor cells (NPCs) viability through the beta-catenin canonical signaling pathway by negatively regulating cell cycle arrest leading to inhibition of neuron apoptotic process (PubMed : 27509850). During hippocampal development, regulates neuroblast proliferation and apoptotic cell death. Controls bone formation through non canonical Wnt signaling mediated via ISG15. Positively regulates bone regeneration through non canonical Wnt signaling (By similarity).
See full target information FZD9
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Product promise

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For full details, please see our Terms & Conditions

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