AB217038

Anti-FZR1 antibody

Name: Anti-FZR1 antibody (ab217038) | Abcam
Brand: Abcam Limited
SKU: AB217038
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal FZR1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FZR1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CDH1, FYR, FZR, KIAA1242, FZR1, Fizzy-related protein homolog, Fzr, CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FZR1 antibody (AB217038)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FZR1 antibody (AB217038)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric cancer tissue labeling FZR1 with ab217038 at 1/200 dilution, followed by secondary antibody detection and DAB staining.

Supplier Data

Western blot - Anti-FZR1 antibody (AB217038)

All lanes:

Western blot - Anti-FZR1 antibody (ab217038) at 1/300 dilution

All lanes:

Mouse intestine lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-FZR1 antibody (AB217038)

All lanes:

Western blot - Anti-FZR1 antibody (ab217038) at 1/300 dilution

All lanes:

Mouse testis lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FZR1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UM11

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FZR1 also known as fizzy-related protein homolog plays a critical mechanical role in cell cycle regulation as a co-activator of the anaphase-promoting complex/cyclosome (APC/C). The FZR1 protein has a mass of about 83 kDa. It is involved in the transition from metaphase to anaphase during cell division. This protein expresses in various tissues but shows higher levels in proliferating cells due to its role in cell cycle control.

Biological function summary

FZR1 helps regulate the degradation of cell cycle proteins by tagging them for destruction in a process involving the ubiquitin-proteasome system. FZR1 forms a complex with APC/C pushing the progression of the cell cycle and ensuring proper chromosome segregation. This activity prevents genomic instability by maintaining the correct order of cell cycle phases.

Pathways

Researchers place FZR1 in the cell cycle pathway particularly in regulating the mitotic exit and G1/S transition. One significant pathway involves its partnership with the cyclin B1 protein where FZR1 aids in its ubiquitination and subsequent degradation. It is also related to pathways involving the retinoblastoma protein (Rb) which adds a layer of regulation over the cycle and ensures DNA replication precision.

FZR1 associates with cancer due to its involvement in controlling cell division and preventing aneuploidy. Disruption in FZR1 function can lead to misregulated cell growth which is often observed in tumors. Furthermore some studies connect FZR1 to neurodegenerative disorders notably through its interactions with proteins in the nervous system although these connections are still under investigation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint : its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed : 25349192).

See full target information FZR1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:198-207 PubMed40739344

2025

Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entry.

Applications

Unspecified application

Species

Unspecified reactive species

Debasish Paul,Derek L Bolhuis,Hualong Yan,Sudipto Das,Xia Xu,Christina C Abbate,Lisa M M Jenkins,Michael J Emanuele,Thorkell Andresson,Jing Huang,John G Albeck,Nicholas G Brown,Steven D Cappell

General physiology and biophysics 41:151-158 PubMed35416178

2022

TRAF3 plays a role in lupus nephritis by regulating Th17 cell and Treg cell balance as well as NF-κB signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Qian Sun,Wen-Si Liu,Hui-Ming Zhang,Chun-Yan Xuan,Yue Wang,Bing-Bing Fu

Cell reports 32:108102 PubMed32905768

2020

The Negative Cross-Talk between SAG/RBX2/ROC2 and APC/C E3 Ligases in Regulation of Cell Cycle Progression and Drug Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Zhang,Yanwen Shen,Hua Li,Chao Bi,Yilun Sun,Xiufang Xiong,Wenyi Wei,Yi Sun

Theranostics 10:9830-9842 PubMed32863962

2020

O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Chu,Mingzuo Jiang,Nan Wu,Bing Xu,Wenjiao Li,Haiming Liu,Song Su,Yanting Shi,Hao Liu,Xiaoliang Gao,Xin Fu,Di Chen,Xiaowei Li,Weijie Wang,Jie Liang,Yongzhan Nie,Daiming Fan

Cell discovery 6:21 PubMed32351703

2020

P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Jing Tian,Chao Huang,Jiao Ma,Gaolei Hu,Yalan Chen,Tianshi Wang,Rong Cai,Yong Zuo,Hongsheng Tan,Qiuju Fan,Baijun Dong,Wei Xue,Jing Yi,Guoqiang Chen,Jun Tu,Jinke Cheng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FZR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FZR1 antibody (AB217038)

Western blot - Anti-FZR1 antibody (AB217038)

Western blot - Anti-FZR1 antibody (AB217038)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise