AB77885

Anti-FZR1 antibody [AR38.2]

KO Validated
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(3 Publications)

Mouse Monoclonal FZR1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FZR1.

View Alternative Names

CDH1, FYR, FZR, KIAA1242, FZR1, Fizzy-related protein homolog, Fzr, CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1

1 Images

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Western blot - Anti-FZR1 antibody [AR38.2] (AB77885)

Lanes 1-4 : Merged signal (red and green). Green - ab77885 observed at 55 kDa. Red - loading control ab181602 observed at 36 kDa.

ab77885 Anti-FZR1 antibody [AR38.2] was shown to specifically react with FZR1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab267263 (knockout cell lysate ab263208) was used. Wild-type and FZR1 knockout samples were subjected to SDS-PAGE. ab77885 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FZR1 antibody [AR38.2] (ab77885) at 1/500 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

FZR1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human FZR1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-fzr1-knockout-hek-293t-cell-line-ab267263'>ab267263</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AR38.2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FZR1.

Q9UM11

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FZR1 also known as fizzy-related protein homolog plays a critical mechanical role in cell cycle regulation as a co-activator of the anaphase-promoting complex/cyclosome (APC/C). The FZR1 protein has a mass of about 83 kDa. It is involved in the transition from metaphase to anaphase during cell division. This protein expresses in various tissues but shows higher levels in proliferating cells due to its role in cell cycle control.

Biological function summary

FZR1 helps regulate the degradation of cell cycle proteins by tagging them for destruction in a process involving the ubiquitin-proteasome system. FZR1 forms a complex with APC/C pushing the progression of the cell cycle and ensuring proper chromosome segregation. This activity prevents genomic instability by maintaining the correct order of cell cycle phases.

Pathways

Researchers place FZR1 in the cell cycle pathway particularly in regulating the mitotic exit and G1/S transition. One significant pathway involves its partnership with the cyclin B1 protein where FZR1 aids in its ubiquitination and subsequent degradation. It is also related to pathways involving the retinoblastoma protein (Rb) which adds a layer of regulation over the cycle and ensures DNA replication precision.

FZR1 associates with cancer due to its involvement in controlling cell division and preventing aneuploidy. Disruption in FZR1 function can lead to misregulated cell growth which is often observed in tumors. Furthermore some studies connect FZR1 to neurodegenerative disorders notably through its interactions with proteins in the nervous system although these connections are still under investigation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint : its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed : 25349192).

See full target information FZR1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413097 PubMed39721007

2024

A Microtubule-Associated Protein Functions in Preventing Oocytes from Evading the Spindle Assembly Checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Changyin Zhou,Xue Zhang,Genlu Xu,Yuting Ran,Hui Wang,Xuefeng Xie,Ang Li,Fei Li,Xiaozhen Li,Jinlong Ding,Mianqun Zhang,Qing-Yuan Sun,Xiang-Hong Ou

Communications biology 4:638 PubMed34050247

2021

Elevating CDCA3 levels in non-small cell lung cancer enhances sensitivity to platinum-based chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina Kildey,Neha S Gandhi,Katherine B Sahin,Esha T Shah,Eric Boittier,Pascal H G Duijf,Christopher Molloy,Joshua T Burgess,Sam Beard,Emma Bolderson,Amila Suraweera,Derek J Richard,Kenneth J O'Byrne,Mark N Adams

Development (Cambridge, England) 137:1297-304 PubMed20223764

2010

Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.

Applications

Species

Mouse

Janet E Holt,Jessica Weaver,Keith T Jones

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FZR1 antibody [AR38.2]

Western blot - Anti-FZR1 antibody [AR38.2] (AB77885)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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