AB93857

Anti-G-6-Pase antibody

Name: Anti-G-6-Pase antibody (ab93857) | Abcam
Brand: Abcam Limited
SKU: AB93857
Price: 390 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal G-6-Pase antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

G6PC, G6PT, G6PC1, Glucose-6-phosphatase catalytic subunit 1, Glucose-6-phosphatase, Glucose-6-phosphatase alpha, G-6-Pase, G6Pase, G6Pase-alpha

1 Images

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Western blot - Anti-G-6-Pase antibody (AB93857)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk blocking buffer before being incubated with ab93857 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

Exposure Time : 12 minutes

All lanes:

Western blot - Anti-G-6-Pase antibody (ab93857) at 1 µg/mL

Lane 1:

HepG2 at 10 µg

Lane 2:

HEK293 at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 40 kDa

Observed band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G-6-Pase also known as glucose-6-phosphatase is an enzyme with a significant role in glucose metabolism. It has a molecular mass of approximately 36 kDa. The enzyme is primarily expressed in the liver and kidneys where it catalyzes the hydrolysis of glucose-6-phosphate into glucose and inorganic phosphate. This reaction is important in the final steps of gluconeogenesis and glycogenolysis processes essential for maintaining blood sugar levels. G-6-Pase is sometimes referred to as the catalytic subunit of the glucose-6-phosphatase complex and is integral for energy homeostasis.

Biological function summary

The enzyme is fundamental in regulating endogenous glucose production. Apart from its standalone activity G-6-Pase works in conjunction with other proteins as part of a larger glucose-6-phosphatase complex located in the endoplasmic reticulum membrane. It interacts with glucose-6-phosphate transporter proteins to efficiently manage glucose output. This complex interplay is important for meeting the organism's energy needs especially during fasted states when glucose supply from dietary intake is low.

Pathways

G-6-Pase plays a major role in gluconeogenesis and glycogenolysis. In gluconeogenesis it helps generate glucose from non-carbohydrate substrates important for energy balance during fasting or exercise. In glycogenolysis it aids in breaking down glycogen into glucose ensuring a constant energy supply. Within these pathways G-6-Pase associates with proteins like glycogen synthase and PEP carboxykinase which coordinate to regulate glucose homeostasis and energy production.

G-6-Pase is tightly linked to glycogen storage disease type I (GSD I) also known as von Gierke disease. This disorder results from a deficiency or dysfunction of the enzyme leading to impaired glucose release and severe hypoglycemia. Additionally misregulation of G-6-Pase activity is implicated in the development of type 2 diabetes where it affects insulin sensitivity and glucose output in the liver. G-6-Pase’s interaction with insulin receptor substrates highlights its connection to these metabolic diseases influencing their progression and severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.

See full target information G6PC1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 28:31-38 PubMed38154962

2024

Lithium and exercise ameliorate insulin-deficient hyperglycemia by independently attenuating pancreatic α-cell mass and hepatic gluconeogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Ryun Jung,Ji-Hye Lee,Hanguk Ryu,Yurong Gao,Jaemin Lee

Immunity, inflammation and disease 11:e969 PubMed38018594

2023

Long noncoding RNA X-inactive specific transcript (lncRNA XIST) inhibits hepatic insulin resistance by competitively binding microRNA-182-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhong,Qingping Yang,Yihua Wang,Yuan Liang,Xiaojing Wang,Dongli Zhao

PloS one 17:e0273701 PubMed36083870

2022

Sexual dimorphism in response to repetitive bouts of acute aerobic exercise in rodents with type 1 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan C Larocque,Silar Gardy,Mitchell Sammut,David P McBey,C W James Melling

Cell metabolism 34:158-170.e5 PubMed34914893

2021

TOX4, an insulin receptor-independent regulator of hepatic glucose production, is activated in diabetic liver.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Wang,Junjie Yu,Qiuzhong Zhou,Xiaobo Wang,Maria Mukhanova,Wen Du,Lei Sun,Utpal B Pajvani,Domenico Accili

Carcinogenesis 42:1196-1207 PubMed34216208

2021

tRNA-derived fragment tRFLys-CTT-010 promotes triple-negative breast cancer progression by regulating glucose metabolism via G6PC.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhu,Jingjing Lu,Xiuling Zhi,Yue Zhou,Xue Wang,Chaofu Wang,Yabiao Gao,Xiufen Zhang,Jerry Yu,Yangbai Sun,Ping Zhou

Nature communications 12:3090 PubMed34035281

2021

mRNA therapy restores euglycemia and prevents liver tumors in murine model of glycogen storage disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jingsong Cao,Minjung Choi,Eleonora Guadagnin,Maud Soty,Marine Silva,Vincent Verzieux,Edward Weisser,Arianna Markel,Jenny Zhuo,Shi Liang,Ling Yin,Andrea Frassetto,Anne-Renee Graham,Kristine Burke,Tatiana Ketova,Cosmin Mihai,Zach Zalinger,Becca Levy,Gilles Besin,Meredith Wolfrom,Barbara Tran,Christopher Tunkey,Erik Owen,Joe Sarkis,Athanasios Dousis,Vladimir Presnyak,Christopher Pepin,Wei Zheng,Lei Ci,Marjie Hard,Edward Miracco,Lisa Rice,Vi Nguyen,Mike Zimmer,Uma Rajarajacholan,Patrick F Finn,Gilles Mithieux,Fabienne Rajas,Paolo G V Martini,Paloma H Giangrande

International journal of molecular sciences 21: PubMed33142766

2020

18F-Fluorodeoxyglucose Positron Emission Tomography Tracks the Heterogeneous Brain Susceptibility to the Hyperglycemia-Related Redox Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Miceli,Vanessa Cossu,Cecilia Marini,Patrizia Castellani,Stefano Raffa,Maria Isabella Donegani,Silvia Bruno,Silvia Ravera,Laura Emionite,Anna Maria Orengo,Federica Grillo,Flavio Nobili,Silvia Morbelli,Antonio Uccelli,Gianmario Sambuceti,Matteo Bauckneht

Cell reports 32:108207 PubMed32997989

2020

Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Wei,Zongshi Lu,Li Li,Hexuan Zhang,Fang Sun,Huan Ma,Lijuan Wang,Yingru Hu,Zhencheng Yan,Hongting Zheng,Gangyi Yang,Daoyan Liu,Martin Tepel,Peng Gao,Zhiming Zhu

International journal of molecular sciences 20: PubMed31744223

2019

The Dichotomous Nature of AZ5104 (an EGFR Inhibitor) Towards RORγ and RORγT.

Applications

Unspecified application

Species

Unspecified reactive species

Kaja Karaś,Anna Sałkowska,Iwona Karwaciak,Aurelia Walczak-Drzewiecka,Jarosław Dastych,Rafał A Bachorz,Marcin Ratajewski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-G-6-Pase antibody

Western blot - Anti-G-6-Pase antibody (AB93857)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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