JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB243319

Anti-G-6-Pase antibody [CL5818]

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse Monoclonal G-6-Pase antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human G6PC1 aa 1-50.

View Alternative Names

G6PC, G6PT, G6PC1, Glucose-6-phosphatase catalytic subunit 1, Glucose-6-phosphatase, Glucose-6-phosphatase alpha, G-6-Pase, G6Pase, G6Pase-alpha

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Formalin-fixed, paraffin-embedded human kidney tissue stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Formalin-fixed, paraffin-embedded human liver tissue stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Negative control :

Formalin-fixed, paraffin-embedded human tonsil tissue lymphoid cells stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL5818

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human G6PC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P35575

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G-6-Pase also known as glucose-6-phosphatase is an enzyme with a significant role in glucose metabolism. It has a molecular mass of approximately 36 kDa. The enzyme is primarily expressed in the liver and kidneys where it catalyzes the hydrolysis of glucose-6-phosphate into glucose and inorganic phosphate. This reaction is important in the final steps of gluconeogenesis and glycogenolysis processes essential for maintaining blood sugar levels. G-6-Pase is sometimes referred to as the catalytic subunit of the glucose-6-phosphatase complex and is integral for energy homeostasis.
Biological function summary

The enzyme is fundamental in regulating endogenous glucose production. Apart from its standalone activity G-6-Pase works in conjunction with other proteins as part of a larger glucose-6-phosphatase complex located in the endoplasmic reticulum membrane. It interacts with glucose-6-phosphate transporter proteins to efficiently manage glucose output. This complex interplay is important for meeting the organism's energy needs especially during fasted states when glucose supply from dietary intake is low.

Pathways

G-6-Pase plays a major role in gluconeogenesis and glycogenolysis. In gluconeogenesis it helps generate glucose from non-carbohydrate substrates important for energy balance during fasting or exercise. In glycogenolysis it aids in breaking down glycogen into glucose ensuring a constant energy supply. Within these pathways G-6-Pase associates with proteins like glycogen synthase and PEP carboxykinase which coordinate to regulate glucose homeostasis and energy production.

G-6-Pase is tightly linked to glycogen storage disease type I (GSD I) also known as von Gierke disease. This disorder results from a deficiency or dysfunction of the enzyme leading to impaired glucose release and severe hypoglycemia. Additionally misregulation of G-6-Pase activity is implicated in the development of type 2 diabetes where it affects insulin sensitivity and glucose output in the liver. G-6-Pase’s interaction with insulin receptor substrates highlights its connection to these metabolic diseases influencing their progression and severity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
See full target information G6PC1
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 13:844148 PubMed35264980

2022

Exogenous Linoleic Acid Intervention Alters Hepatic Glucose Metabolism in an Avian Embryo Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufen Zhang,Qilin Wu,Wenxuan Zheng,Chuang Liu,Liang Huang,Xin Zuo,Wenquan Xiao,Xiaofeng Han,Hui Ye,Wence Wang,Yongwen Zhu,Lin Yang

Theranostics 11:7829-7843 PubMed34335967

2021

MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zheng,Jian Wan,Yi Shan,Xi Song,Jie Jin,Qing Su,Song Chen,Xinyuan Lu,Jialin Yang,Quanmin Li,Yuping Song,Bo Li

Journal of cellular and molecular medicine : PubMed32567187

2020

Large-scale transcriptome profiles reveal robust 20-signatures metabolic prediction models and novel role of G6PC in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hao Xu,Yue Xu,Xi Tian,Aihetaimujiang Anwaier,Wang-Rui Liu,Jun Wang,Wen-Kai Zhu,Da-Long Cao,Hong-Kai Wang,Guo-Hai Shi,Yuan-Yuan Qu,Hai-Liang Zhang,Ding-Wei Ye
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB93857

Anti-G-6-Pase antibody

Western blot - Anti-G-6-Pase antibody (AB93857)

  • 3
  • 1

Complementary Products

Primary Antibodies

AB187145

Anti-PCK2 antibody [EPR14224] - C-terminal

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)

  • 4
  • 1
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB88056

Anti-Glucokinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glucokinase antibody (AB88056)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com