Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)

Rabbit Recombinant Monoclonal G-6-Pase antibody. Carrier free. Suitable for Dot, I-ELISA, IHC-P, ICC/IF, WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (AB319054), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	I-ELISA	IHC-P	ICC/IF	WB	IP	Flow Cyt (Intra)
Human	Expected	Expected	Tested	Tested	Tested	Tested	Not recommended
Mouse	Expected	Expected	Tested	Expected	Tested	Tested	Not recommended
Rat	Expected	Expected	Tested	Expected	Tested	Expected	Not recommended
Recombinant fragment - Human	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Synthetic peptide - Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human, Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human, Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human, Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human, Synthetic peptide - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human, Synthetic peptide - Human	Dilution info -	Notes -

Target data

See full target information Glucose-6-phosphatase

Function

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.

Alternative names

G6PC, G6PT, G6PC1, Glucose-6-phosphatase catalytic subunit 1, Glucose-6-phosphatase, Glucose-6-phosphatase alpha, G-6-Pase, G6Pase, G6Pase-alpha

Recommended products

Rabbit Recombinant Monoclonal G-6-Pase antibody. Carrier free. Suitable for Dot, I-ELISA, IHC-P, ICC/IF, WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28793-52
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab319054 is the carrier-free version of Anti-G-6-Pase antibody [EPR28793-52] ab319053.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

G-6-Pase also known as glucose-6-phosphatase is an enzyme with a significant role in glucose metabolism. It has a molecular mass of approximately 36 kDa. The enzyme is primarily expressed in the liver and kidneys where it catalyzes the hydrolysis of glucose-6-phosphate into glucose and inorganic phosphate. This reaction is important in the final steps of gluconeogenesis and glycogenolysis processes essential for maintaining blood sugar levels. G-6-Pase is sometimes referred to as the catalytic subunit of the glucose-6-phosphatase complex and is integral for energy homeostasis.

Biological function summary

The enzyme is fundamental in regulating endogenous glucose production. Apart from its standalone activity G-6-Pase works in conjunction with other proteins as part of a larger glucose-6-phosphatase complex located in the endoplasmic reticulum membrane. It interacts with glucose-6-phosphate transporter proteins to efficiently manage glucose output. This complex interplay is important for meeting the organism's energy needs especially during fasted states when glucose supply from dietary intake is low.

Pathways

G-6-Pase plays a major role in gluconeogenesis and glycogenolysis. In gluconeogenesis it helps generate glucose from non-carbohydrate substrates important for energy balance during fasting or exercise. In glycogenolysis it aids in breaking down glycogen into glucose ensuring a constant energy supply. Within these pathways G-6-Pase associates with proteins like glycogen synthase and PEP carboxykinase which coordinate to regulate glucose homeostasis and energy production.

Associated diseases and disorders

G-6-Pase is tightly linked to glycogen storage disease type I (GSD I) also known as von Gierke disease. This disorder results from a deficiency or dysfunction of the enzyme leading to impaired glucose release and severe hypoglycemia. Additionally misregulation of G-6-Pase activity is implicated in the development of type 2 diabetes where it affects insulin sensitivity and glucose output in the liver. G-6-Pase’s interaction with insulin receptor substrates highlights its connection to these metabolic diseases influencing their progression and severity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human liver.

The section was incubated with Anti-G-6-Pase antibody [EPR28793-52] ab319053 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Indirect ELISA - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human G-6-Pase.
Antigen concentration: 1000 ng/ml
Dot Blot - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Dot blot analysis of G-6-Pase using Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: His tagged human G-6-Pase protein

Lane 2: Human G6PC2 peptide
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human G6PC2.
All lanes: Dot Blot - Anti-G-6-Pase antibody [EPR28793-52] (Anti-G-6-Pase antibody [EPR28793-52] ab319053) at 1/1000 dilution
Lane 1: His tagged human G-6-Pase protein
Lane 2: Human G6PC2 peptide
Secondary
All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Immunoprecipitation - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
G-6-Pase was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver tissue lysate

Lane 2: Anti-G-6-Pase antibody [EPR28793-52] ab319053 IP in Mouse liver tissue lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-G-6-Pase antibody [EPR28793-52] ab319053 in mouse liver tissue lysate
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-G-6-Pase antibody [EPR28793-52] (Anti-G-6-Pase antibody [EPR28793-52] ab319053) at 1/30 dilution
All lanes: Mouse liver tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
Immunoprecipitation - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
G-6-Pase was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Lane 2: Anti-G-6-Pase antibody [EPR28793-52] ab319053 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-G-6-Pase antibody [EPR28793-52] ab319053 in HepG2 whole cell lysate
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-G-6-Pase antibody [EPR28793-52] (Anti-G-6-Pase antibody [EPR28793-52] ab319053) at 1/30 dilution
All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
Western blot - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle, spleen(PMID: 9822626), pancreas
The identity of the higher MW band at approximately 50 kDa is unknown
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-G-6-Pase antibody [EPR28793-52] (Anti-G-6-Pase antibody [EPR28793-52] ab319053) at 1/1000 dilution
Lane 1: Rat liver tissue lysate at 20 µg
Lane 2: Rat kidney tissue lysate at 20 µg
Lane 3: Rat skeletal muscle tissue at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
Lane 5: Rat pancreas tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 36 kDa
Exposure time: 26s
Western blot - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle, spleen(PMID: 9822626), pancreas
The identity of the higher MW bands above 36 kDa are unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-G-6-Pase antibody [EPR28793-52] (Anti-G-6-Pase antibody [EPR28793-52] ab319053) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Mouse pancreas tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 36 kDa
Exposure time: 26s
Western blot - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle, spleen, THP-1, JEG-3, U-937(PMID: 9822626), pancreas
The identity of the higher MW band at approximately 50 kDa is unknown
The identity of the lower MW band at approximately 20 kDa is unknown
The samples of lane2-8 are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-G-6-Pase antibody [EPR28793-52] (Anti-G-6-Pase antibody [EPR28793-52] ab319053) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
Lane 4: Human spleen tissue lysate at 20 µg
Lane 5: Human pancreas tissue lysate at 20 µg
Lane 6: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 7: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 8: JEG-3 (human placenta epithelial cell) whole cell lysate at 20 µg
Lane 9: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 36 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat liver (PMID: 9813078).

The section was incubated with Anti-G-6-Pase antibody [EPR28793-52] ab319053 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems Bond™ Polymer Refine Detection

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat skeletal muscle.

The section was incubated with Anti-G-6-Pase antibody [EPR28793-52] ab319053 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human kidney.

The section was incubated with Anti-G-6-Pase antibody [EPR28793-52] ab319053 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/50 (10.02 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 μg/ml dilution (Green).
Confocal image showing cytoplasmic staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

Negative control: THP-1 (PMID: 9822626).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).
Positive staining on mouse liver (PMID: 29428299, 25147289).

The section was incubated with Anti-G-6-Pase antibody [EPR28793-52] ab319053 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human skeletal muscle.

The section was incubated with Anti-G-6-Pase antibody [EPR28793-52] ab319053 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [EPR28793-52] - BSA and Azide free (ab319054)
This data was developed using Anti-G-6-Pase antibody [EPR28793-52] ab319053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling G-6-Pase with Anti-G-6-Pase antibody [EPR28793-52] ab319053 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse skeletal muscle.

The section was incubated with Anti-G-6-Pase antibody [EPR28793-52] ab319053 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins