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AB204989

Anti-G-CSF antibody [7E4F7]

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(3 Publications)

Mouse Monoclonal G-CSF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CSF3.

View Alternative Names

C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [7E4F7] (AB204989)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [7E4F7] (AB204989)

Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling G-CSF with ab204989 at 1/200 dilution with DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (AB204989)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (AB204989)

Immunofluorescent analysis of HeLa cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (AB204989)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (AB204989)

Immunofluorescent analysis of A549 cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Western blot - Anti-G-CSF antibody [7E4F7] (AB204989)
  • WB

Supplier Data

Western blot - Anti-G-CSF antibody [7E4F7] (AB204989)

All lanes:

Western blot - Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 22 kDa

false

Western blot - Anti-G-CSF antibody [7E4F7] (AB204989)
  • WB

Supplier Data

Western blot - Anti-G-CSF antibody [7E4F7] (AB204989)

Expected MWt is 48.2 kDa.

All lanes:

Western blot - Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution

All lanes:

Human G-CSF (aa1-207) recombinant protein

Predicted band size: 22 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7E4F7

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CSF3.

P09919

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
See full target information CSF3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 53:102310 PubMed39929064

2025

CSF3 promotes colorectal cancer progression by activating p65/NF-κB signaling pathway and inducing an immunosuppressive microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Xu,Na Li,Hui Xie,Changwei Duan,Xingchen Liao,Ruoran Li,Heng Zhang,Yuanming Pan,Xianzong Ma,Shuwen Du,Jianqiu Sheng,Xin Wang,Lang Yang,Peng Jin

The Journal of cell biology 223: PubMed39150509

2024

Nuclear poly-glutamine aggregates rupture the nuclear envelope and hinder its repair.

Applications

Unspecified application

Species

Unspecified reactive species

Giel Korsten,Miriam Osinga,Robin A Pelle,Albert K Serweta,Baukje Hoogenberg,Harm H Kampinga,Lukas C Kapitein

Frontiers in cell and developmental biology 8:601043 PubMed33415106

2020

Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling Induction of G-CSF.

Applications

Unspecified application

Species

Unspecified reactive species

Joy Yue Zhang,Peihuang Wu,Danyang Chen,Fen Ning,Qinsheng Lu,Xiu Qiu,Martin Hewison,Jennifer A Tamblyn,Mark D Kilby,Gendie E Lash
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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