Rabbit Polyclonal G-CSF antibody. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus.
IgG
Rabbit
Standard buffer
Lyophilized
Polyclonal
WB | sELISA | |
---|---|---|
Human | Tested | Expected |
Recombinant full length protein - Human | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
C17orf33, GCSF, CSF3, C17orf33, GCSF, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin
Rabbit Polyclonal G-CSF antibody. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus.
IgG
Rabbit
Standard buffer
Lyophilized
Polyclonal
Affinity purification Immunogen
unknown
reconstitute with water at 200µL
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see Anti-G-CSF antibody [CSF3/900] ab216607.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Standard Curve for G-CSF (Analyte: Recombinant human G-CSF protein ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml
All lanes: Western blot - Anti-G-CSF antibody (ab9691) at 1 µg/mL
All lanes: Recombinant human G-CSF protein (ab73371) at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Exposure time: 2min
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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