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Rabbit Polyclonal G-CSF antibody. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus.


Images

Sandwich ELISA - Anti-G-CSF antibody (AB9691), expandable thumbnail
  • Western blot - Anti-G-CSF antibody (AB9691), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Standard buffer

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09919

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBsELISA
Human
Tested
Expected
Recombinant full length protein - Human
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

0.5-2 µg/mL

Notes

This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

12 products for Alternative Product

Target data

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Alternative names

Recommended products

Rabbit Polyclonal G-CSF antibody. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus.

Key facts

Isotype

IgG

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09919
Purification technique

Affinity purification Immunogen

Light chain type

unknown

Reconstitution

reconstitute with water at 200µL

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see Anti-G-CSF antibody [CSF3/900] ab216607.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Sandwich ELISA - Anti-G-CSF antibody (ab9691), expandable thumbnail

    Sandwich ELISA - Anti-G-CSF antibody (ab9691)

    Standard Curve for G-CSF (Analyte: Recombinant human G-CSF protein ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml

  • Western blot - Anti-G-CSF antibody (ab9691), expandable thumbnail

    Western blot - Anti-G-CSF antibody (ab9691)

    All lanes: Western blot - Anti-G-CSF antibody (ab9691) at 1 µg/mL

    All lanes: Recombinant human G-CSF protein (ab73371) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Exposure time: 2min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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