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AB9691

Anti-G-CSF antibody

4

(6 Reviews)

|

(15 Publications)

Rabbit Polyclonal G-CSF antibody. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus.

View Alternative Names

C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin

2 Images
Sandwich ELISA - Anti-G-CSF antibody (AB9691)
  • sELISA

Unknown

Sandwich ELISA - Anti-G-CSF antibody (AB9691)

Standard Curve for G-CSF (Analyte : ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml

Western blot - Anti-G-CSF antibody (AB9691)
  • WB

Unknown

Western blot - Anti-G-CSF antibody (AB9691)

All lanes:

Western blot - Anti-G-CSF antibody (ab9691) at 1 µg/mL

All lanes:

Recombinant human G-CSF protein (<a href='/en-us/products/unavailable/recombinant-human-g-csf-protein-ab73371'>ab73371</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 22 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human CSF3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09919

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5-2 µg/mL", "sELISA-species-notes": "<p>This antigen affinity purified antibody, in conjunction with <a href='/en-us/products/primary-antibodies/biotin-g-csf-antibody-ab271256'>ab271256</a> as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF</p>" } } }

Product details

Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab216607.

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
See full target information CSF3

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Case reports in oncology 17:1115-1123 PubMed39474544

2024

A Case of Granulocyte-Colony-Stimulating Factor-Producing Non-Small Cell Lung Cancer under Steroid Treatment and with Poor Performance Status That Responded to Pembrolizumab.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Egusa,Takeshi Masuda,Kakuhiro Yamaguchi,Shinjiro Sakamoto,Yasushi Horimasu,Kei Kushitani,Taku Nakashima,Hiroshi Iwamoto,Hironobu Hamada,Noboru Hattori

Genes to cells : devoted to molecular & cellular mechanisms 28:573-584 PubMed37248626

2023

Squamous cell carcinoma-derived G-CSF promotes tumor growth and metastasis in mice through neutrophil recruitment and tumor cell proliferation, associated with poor prognosis of the patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kohei Kemuriyama,Jianbo An,Satoru Motoyama,Yushi Nagaki,Tomokazu Yamaguchi,Yusuke Sato,Akiyuki Wakita,Yoshihiro Minamiya,Keiji Kuba

Clinical cancer research : an official journal of the American Association for Cancer Research 29:2158-2169 PubMed36951682

2023

G-CSF Is a Novel Mediator of T-Cell Suppression and an Immunotherapeutic Target for Women with Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anita L Ray,Apryl S Saunders,Robert A Nofchissey,Megan A Reidy,Maria Kamal,Megan R Lerner,Kar-Ming Fung,Mark L Lang,Joshua A Hanson,Shaoxuan Guo,Maria G Urdaneta-Perez,Samara E Lewis,Michael Cloyde,Katherine T Morris

Histology and histopathology 37:985-997 PubMed35543246

2022

Knockdown of circRNA circEBF1 inhibits cutaneous squamous cell carcinoma progression by regulating the miR-1247-5p/CSF3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Yin,Guo Wei,Xiaoyan Sun,Yan Yin

Archives of gynecology and obstetrics 304:985-997 PubMed33608803

2021

Methotrexate-induced toxic effects and the ameliorating effects of astaxanthin on genitourinary tissues in a female rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Ilker Gunyeli,Mustafa Saygin,Ozlem Ozmen

Frontiers in cell and developmental biology 8:601043 PubMed33415106

2020

Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling Induction of G-CSF.

Applications

Unspecified application

Species

Unspecified reactive species

Joy Yue Zhang,Peihuang Wu,Danyang Chen,Fen Ning,Qinsheng Lu,Xiu Qiu,Martin Hewison,Jennifer A Tamblyn,Mark D Kilby,Gendie E Lash

Journal of molecular cell biology 12:723-737 PubMed32242230

2020

Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Yudong Wu,Cheng Zhang,Chong Zhou,Yining Li,Yi Zeng,Chunbo Zhang,Rong Li,Daya Luo,Lieliang Wang,Long Zhang,Shuo Tu,Huan Deng,Shiwen Luo,Ye-Guang Chen,Xiangyang Xiong,Xiaohua Yan

Journal of biochemical and molecular toxicology 34:e22445 PubMed31975554

2020

Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rahime Aslankoc,Ozlem Ozmen,Hamit Yasar Ellidag

Analytical chemistry 91:11004-11012 PubMed31361950

2019

Wood Microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Abhay Andar,Md-Sadique Hasan,Venkatesh Srinivasan,Mustafa Al-Adhami,Erick Gutierrez,David Burgenson,Xudong Ge,Leah Tolosa,Yordan Kostov,Govind Rao

Breast cancer research and treatment 175:459-472 PubMed30778902

2019

A high-risk luminal A dominant breast cancer subtype with increased mobility.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Guo,Guoji Chen,Wen Zhang,Lulin Zhou,Ting Xiao,Xuebing Di,Yipeng Wang,Lin Feng,Kaitai Zhang
View all publications

Product promise

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