Rabbit Recombinant Monoclonal G-CSF antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 13 publications.
Images
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--western-blot-img9612.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053)")
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunoprecipitation-img323.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053)")
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunocytochemistry-immunofluorescence-img14822.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053)")
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--western-blot-img104915.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053)")
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunoprecipitation-img13906.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053)")
Publications
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- Cellular and molecular life sciences : CMLS 80:1842023Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiju Ye,He Huang,Yun Xiao,Xue Han,Fengjie Shi,Wu Luo,Jiawen Chen,Yang Ye,Xia Zhao,Weijian Huang,Yi Wang,Dongwu Lai,Guang Liang,Guosheng FuPubMed 37340199
- Genes to cells : devoted to molecular & cellular mechanisms 28:573-5842023Squamous cell carcinoma-derived G-CSF promotes tumor growth and metastasis in mice through neutrophil recruitment and tumor cell proliferation, associated with poor prognosis of the patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKohei Kemuriyama,Jianbo An,Satoru Motoyama,Yushi Nagaki,Tomokazu Yamaguchi,Yusuke Sato,Akiyuki Wakita,Yoshihiro Minamiya,Keiji KubaPubMed 37248626
- Cancer gene therapy 29:1742-17502022Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobin Wang,Xiaoyu Liu,Huihan WangPubMed 35768562
- Frontiers in microbiology 13:8377762022Suppression of Germination by Neutrophils Is Enhanced by Endothelial-Derived CSF3 Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenxin Zhang,Dan He,Yunyun Wei,Shumi Shang,Dong Li,Li WangPubMed 35572651
- Oxidative medicine and cellular longevity 2022:48772752022Ruscogenins Improve CD-Like Enteritis by Inhibiting Apoptosis of Intestinal Epithelial Cells and Activating Nrf2/NQO1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHexin Wen,Xiaofeng Zhang,Qingqing Li,Ju Huang,Guangyong Liu,Jingyue Zhao,Yiran Liu,Li Shen,Yuyang Li,Kun Yang,Lugen Zuo,Jing Li,Jing Nian,Ping Xiang,Hao Zhao,Liang Yu,Mulin Liu,Zhijun Geng,Xue SongPubMed 35308175
- The Journal of investigative dermatology 142:692-704.e142021IL-10 Dysregulation Underlies Chemokine Insufficiency, Delayed Macrophage Response, and Impaired Healing in Diabetic Wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuchi Roy,Janet Zayas,Mohamed F Mohamed,Anahita Aboonabi,Kaylee Delgado,Jennillee Wallace,Mohammad Bayat,Timothy M Kuzel,Jochen Reiser,Sasha H ShafikhaniPubMed 34517005
- BMC oral health 21:2992021Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Yu,Tianyi Zhang,Haibin Lu,Qi Ma,Dong Zhao,Jiang Sun,Zuomin WangPubMed 34118920
- Frontiers in physiology 11:6057922021CSF3 Is a Potential Drug Target for the Treatment of COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Fang,Jie Mei,Huixiang Tian,Yu-Ligh Liou,Dingchao Rong,Wei Zhang,Qianjin Liao,Nayiyuan WuPubMed 33551833
- Molecular & cellular proteomics : MCP 19:793-8072020The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivier Touzelet et. al.PubMed 32075873
- Cell death & disease 9:5782018Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Wang et. al.PubMed 29784961
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes For unpurified use at 1/20 dilution. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/50 - 1/100 dilution. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
1 product for Alternative Version
Target data
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Alternative names
C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin
Recommended products
Rabbit Recombinant Monoclonal G-CSF antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3203(N)(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--western-blot-img9612.jpg "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 4 s
All lanes: Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/1000 dilution
Lane 1: A549 (Human lung carcinoma epithelial cell) cell lysate at 10 µg
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate at 10 µg
Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 10 µg
Lane 5: MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate at 10 µg
Lane 6: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate at 10 µg
Lane 7: PC-3 (Human prostate adenocarcinoma epithelial cell) cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunoprecipitation-img323.jpg "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
ab181053 (purified) at 1:100 dilution (2ug) immunoprecipitating G-CSF in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10ug
Lane 2 (+): ab181053 & K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab181053 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST."All lanes: Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Predicted band size: 22 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunocytochemistry-immunofluorescence-img14822.jpg "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cells labeling G-CSF with Purified ab181053 at 1:500 dilution (4.0μg/ml). Cells were fixed in 100% Methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear was used as a counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--western-blot-img104915.jpg "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
All lanes: Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/2000 dilution
Lane 1: K562 cell lysate at 20 µg
Lane 2: KM3 cell lysate at 20 µg
Lane 3: NCI-H460 cell lysate at 20 µg
Lane 4: HT-1376 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Predicted band size: 22 kDa
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunoprecipitation-img13906.jpg "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Western blot analysis of immunoprecipitation pellet from K562 cell lysate (lane 1) or a Negative control (lane 2) immunoprecipitated using unpurified ab181053 at 1/20 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
All lanes: Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Predicted band size: 22 kDa
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--western-blot-img159511.jpg "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/2000 dilution
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg
Lane 2: HT-1376 (Human urinary bladder carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3: Mouse brain lysates at 20 µg
Lane 4: Rat brain lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunocytochemistry-immunofluorescence-img89215.jpg "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling G-CSF with unpurified ab181053 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--flow-cytometry-intracellular-img415533.jpg "Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
Intracellular Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling G-CSF with purified ab181053 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--western-blot-img159511.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--immunocytochemistry-immunofluorescence-img89215.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
![Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-ab181053--flow-cytometry-intracellular-img415533.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)")
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