Rabbit Recombinant Monoclonal G-CSF antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--western-blot-img9612.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157)")
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunoprecipitation-img323.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157)")
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunocytochemistry-immunofluorescence-img14822.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157)")
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunoprecipitation-img13906.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157)")
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunocytochemistry-immunofluorescence-img89215.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (AB236157)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
2 products for Alternative Version
Target data
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Alternative names
C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin
Recommended products
Rabbit Recombinant Monoclonal G-CSF antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3203(N)(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236157 is the carrier-free version of Anti-G-CSF antibody [EPR3203(N)(B)] ab181053.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--western-blot-img9612.jpg "Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)")
Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 4 s
All lanes: Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053) at 1/1000 dilution
Lane 1: A549 (Human lung carcinoma epithelial cell) cell lysate at 10 µg
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate at 10 µg
Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 10 µg
Lane 5: MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate at 10 µg
Lane 6: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate at 10 µg
Lane 7: PC-3 (Human prostate adenocarcinoma epithelial cell) cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunoprecipitation-img323.jpg "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)")
Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Anti-G-CSF antibody [EPR3203(N)(B)] ab181053 (purified) at 1:100 dilution (2μg) immunoprecipitating G-CSF in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg
Lane 2 (+): Anti-G-CSF antibody [EPR3203(N)(B)] ab181053 and K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-G-CSF antibody [EPR3203(N)(B)] ab181053 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053).
All lanes: Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053)
Predicted band size: 22 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunocytochemistry-immunofluorescence-img14822.jpg "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)")
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cells labeling G-CSF with purified Anti-G-CSF antibody [EPR3203(N)(B)] ab181053 at 1:500 dilution (4.0μg/ml). Cells were fixed in 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear was used as a counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053).
![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunoprecipitation-img13906.jpg "Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)")
Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Western blot analysis of immunoprecipitation pellet from K562 cell lysate (lane 1) or a Negative control (lane 2) immunoprecipitated using unpurified Anti-G-CSF antibody [EPR3203(N)(B)] ab181053 at 1/20 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053).
All lanes: Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053)
Predicted band size: 22 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--immunocytochemistry-immunofluorescence-img89215.jpg "Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)")
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling G-CSF with unpurified Anti-G-CSF antibody [EPR3203(N)(B)] ab181053 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053).
![Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--flow-cytometry-intracellular-img415533.jpg "Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)")
Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Intracellular Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling G-CSF with purified Anti-G-CSF antibody [EPR3203(N)(B)] ab181053 at 1/200 dilution (10μg/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-G-CSF antibody [EPR3203(N)(B)] ab181053).
![Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157), expandable thumbnail](https://content.abcam.com/products/images/g-csf-antibody-epr3203nb-bsa-and-azide-free-ab236157--flow-cytometry-intracellular-img415533.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)")
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