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AB154698

Anti-G gamma12 antibody

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(3 Publications)

Rabbit Polyclonal G gamma12 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 aa 1 to C-terminus.

View Alternative Names

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12, GNG12

1 Images
Western blot - Anti-G gamma12 antibody (AB154698)
  • WB

Unknown

Western blot - Anti-G gamma12 antibody (AB154698)

15% SDS PAGE

All lanes:

Western blot - Anti-G gamma12 antibody (ab154698) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 8 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBI6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The G gamma12 protein also known as guanine nucleotide-binding protein subunit gamma 12 is a component of the heterotrimeric G proteins. It has an approximate molecular mass of 8 kDa. This protein predominantly resides at the plasma membrane where it partners with its associated G alpha and G beta subunits. G gamma12 shows expression in various tissues including the brain and heart where it functions as an integral part of signal transduction processes.
Biological function summary

G gamma12 is involved in transmitting signals within cells by interacting with its G alpha and G beta counterparts to form a functional G protein complex. This complex plays a critical role in modulating and transducing signals from activated cell surface receptors to downstream effectors. As a member of the G protein gamma subunit family G gamma12 aids in diverse cellular responses such as cell growth differentiation and motility.

Pathways

G gamma12 plays a part in the MAPK signaling pathway and the PI3K-Akt signaling pathway. These pathways are significant for regulating various cellular activities like survival and metabolism. Proteins like G beta and specific G alpha subunits closely interact with G gamma12 in these pathways influencing the downstream signaling cascades and cellular responses.

G gamma12 has associations with cancer and cardiovascular diseases. In the context of cancer altered G gamma12 expression affects cell proliferation and migration thereby contributing to tumor development. In cardiovascular diseases it implicates processes such as heart rate regulation. Through these conditions G gamma12 connects to other proteins like G alpha q and beta-adrenergic receptors contributing to the disease state's pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
See full target information Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Diabetes 73:2045-2054 PubMed39320956

2024

Optimized Proteomic Analysis of Insulin Granules From MIN6 Cells Identifies Scamp3, a Novel Regulator of Insulin Secretion and Content.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Norris,Belinda Yau,Carlo Famularo,Hayley Webster,Thomas Loudovaris,Helen E Thomas,Mark Larance,Alistair M Senior,Melkam A Kebede

Journal of clinical laboratory analysis 36:e24207 PubMed35060646

2022

Circular RNA hsa_circ_0000285 regulates the microRNA-599/G-protein subunit gamma 12 (miR-599/GNG12) axis to promote glioma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Liu,Chen Duan,Ya Han

FEBS open bio : PubMed31898405

2020

GNG12 regulates PD-L1 expression by activating NF-κB signaling in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Li,Can Jin,Chuanxin Zou,Xu Qiao,Peng Ma,Di Hu,Wenqin Li,Jun Jin,Xin Jin,Ping Fan
View all publications
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Product promise

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