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AB204757

Anti-G gamma12 antibody

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(1 Publication)

Rabbit Polyclonal G gamma12 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 aa 1-50.

View Alternative Names

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12, GNG12

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-G gamma12 antibody (AB204757)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-G gamma12 antibody (AB204757)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling G gamma12 in the mitochondria with ab204757 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G gamma12 antibody (AB204757)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G gamma12 antibody (AB204757)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling G gamma12 (low expression) with ab204757 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G gamma12 antibody (AB204757)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G gamma12 antibody (AB204757)

Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling G gamma12 (high expression) with ab204757 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UBI6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The G gamma12 protein also known as guanine nucleotide-binding protein subunit gamma 12 is a component of the heterotrimeric G proteins. It has an approximate molecular mass of 8 kDa. This protein predominantly resides at the plasma membrane where it partners with its associated G alpha and G beta subunits. G gamma12 shows expression in various tissues including the brain and heart where it functions as an integral part of signal transduction processes.
Biological function summary

G gamma12 is involved in transmitting signals within cells by interacting with its G alpha and G beta counterparts to form a functional G protein complex. This complex plays a critical role in modulating and transducing signals from activated cell surface receptors to downstream effectors. As a member of the G protein gamma subunit family G gamma12 aids in diverse cellular responses such as cell growth differentiation and motility.

Pathways

G gamma12 plays a part in the MAPK signaling pathway and the PI3K-Akt signaling pathway. These pathways are significant for regulating various cellular activities like survival and metabolism. Proteins like G beta and specific G alpha subunits closely interact with G gamma12 in these pathways influencing the downstream signaling cascades and cellular responses.

G gamma12 has associations with cancer and cardiovascular diseases. In the context of cancer altered G gamma12 expression affects cell proliferation and migration thereby contributing to tumor development. In cardiovascular diseases it implicates processes such as heart rate regulation. Through these conditions G gamma12 connects to other proteins like G alpha q and beta-adrenergic receptors contributing to the disease state's pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
See full target information Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:22028 PubMed39322716

2024

Autologous micrografting improves regeneration of tissue-engineered urinary conduits in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolai Juul,Mahboobeh Amoushahi,Oliver Willacy,Micki Ji,Chiara Villa,Fatemeh Ajalloueian,Clara Chamorro,Magdalena Fossum
View all publications
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