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AB264140

Anti-G protein beta subunit like/GBL antibody

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Rabbit Polyclonal G protein beta subunit like/GBL antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MLST8 aa 250-350.

View Alternative Names

GBL, LST8, MLST8, Target of rapamycin complex subunit LST8, TORC subunit LST8, G protein beta subunit-like, Mammalian lethal with SEC13 protein 8, Gable, Protein GbetaL, mLST8

1 Images
Immunoprecipitation - Anti-G protein beta subunit like/GBL antibody (AB264140)
  • IP

Supplier Data

Immunoprecipitation - Anti-G protein beta subunit like/GBL antibody (AB264140)

G protein beta subunit like/GBL was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264140 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264140 at 1 μg/ml.

Lane 1 : ab264140 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 3 mins.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-G protein beta subunit like/GBL antibody (ab264140)

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human MLST8 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q9BVC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The G protein beta subunit-like protein often known as GBL is an essential component in cellular signaling. It plays a mechanical role as part of the larger G protein complex that transduces signals from cell surface receptors to intracellular pathways. Though we do not have a precise molecular weight for GBL it is known to interact closely with other subunits of the G protein complex. GBL is widely expressed across various tissues reflecting its involvement in numerous cellular processes.
Biological function summary

GBL contributes to the regulation and setting up of signal transduction pathways by forming a part of the G protein complex. This complex involves the alpha beta and gamma subunits where GBL typically interacts with the beta subunit family. By binding to receptors like GPCRs GBL facilitates the exchange of GDP for GTP on the G protein alpha subunit thereby activating downstream effectors. The complex is critical in ensuring precise cellular responses and GBL maintains structural integrity within it.

Pathways

GBL plays an integral role in the G protein-coupled receptor (GPCR) signaling and the MAPK signaling pathways. In GPCR signaling GBL's interaction with the beta subunit influences processes like sensory perception and cell growth. In the MAPK pathway critical for cell responses to growth signals GBL connects with proteins such as RAF and MEK supporting the cascade of phosphorylation events critical for pathway progression. These pathways highlight GBL's importance in cellular dynamics and network interactions.

Scientists associate GBL with certain cancers and metabolic disorders. Aberrant expression or dysfunction of GBL can lead to disrupted signal transduction an important feature in pathways involved in tumorigenesis such as those involving GPCR signaling anomalies. Additionally the MAPK pathway's misregulation where GBL plays a part links to metabolic disorders. This makes GBL a potential therapeutic target offering insights into developing treatments by targeting associated proteins like GPCRs and components of the MAPK pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals (PubMed : 12718876, PubMed : 15268862, PubMed : 15467718, PubMed : 24403073, PubMed : 28489822). mTORC1 is activated in response to growth factors or amino acids (PubMed : 12718876, PubMed : 15268862, PubMed : 15467718, PubMed : 24403073). In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) (PubMed : 12718876, PubMed : 15268862, PubMed : 15467718, PubMed : 24403073). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy (PubMed : 24403073). The mTORC1 complex is inhibited in response to starvation and amino acid depletion (PubMed : 24403073). Within mTORC1, MLST8 interacts directly with MTOR and enhances its kinase activity (PubMed : 12718876). In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity (PubMed : 12718876). As part of the mTORC2 complex, transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output (PubMed : 15467718, PubMed : 35926713). mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive (PubMed : 15467718, PubMed : 35926713). In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1 (PubMed : 15467718, PubMed : 35926713). mTORC2 functions upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed : 15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed : 15467718). mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation (PubMed : 15467718). mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed : 15467718). mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657' (PubMed : 15467718). Within mTORC2, MLST8 acts as a bridge between MAPKAP1/SIN1 and MTOR (PubMed : 31085701).
See full target information MLST8
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