Anti-G-protein coupled receptor 30 antibody - C-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunofluorescence analysis of Mock and transfected 293T cells fixed in ice-cold MeOH for 5 min labelling GPR30 protein using ab154069 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue stained for GPR30 protein at cytoplasm with ab154069 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue stained for GPR30 protein at cell membrane and cytoplasm with ab154069 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunofluorescence analysis of DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling GPR30 protein using ab154069 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, stained by a beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunohistochemical analysis of paraffin-embedded mouse tissues stained for GPR30 protein with ab154069 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Immunofluorescence analysis of DIV11 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling GPR30 protein using ab154069 at a 1/100 dilution (Green). beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by a beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

• WB

Supplier Data

Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/5000 dilution

Lane 1:

Non-transfected and boiled 293T whole cell extracts at 30 µg

Lane 2:

Transfected and boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected and unboiled 293T whole cell extracts at 30 µg

Lane 4:

Transfected and unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 42 kDa

true

• WB

Supplier Data

Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/1000 dilution

Lane 1:

Boiled mouse stomach tissue extract at 50 µg

Lane 2:

Boiled mouse liver tissue extract at 50 µg

Lane 3:

Unboiled mouse stomach tissue extract at 50 µg

Lane 4:

Unboiled mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 42 kDa

true

• WB

Supplier Data

Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (AB154069)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/1000 dilution

Lane 1:

Boiled AsPC-1 whole cell extracts at 30 µg

Lane 2:

Boiled AsPC-1 membrane extracts at 30 µg

Lane 3:

Unboiled AsPC-1 whole cell extracts at 30 µg

Lane 4:

Unboiled AsPC-1 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 42 kDa

true