Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033) is a rabbit monoclonal antibody detecting G-protein coupled receptor 30 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
Images
![Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--western-blot-img8100.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99161.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76522.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120423.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44698.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033)")
Publications
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- Nature communications 14:69912023Follicle-stimulating hormone orchestrates glucose-stimulated insulin secretion of pancreatic islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Cheng,Hong Zhu,Jun Ren,Hai-Yan Wu,Jia-En Yu,Lu-Yang Jin,Hai-Yan Pang,Hai-Tao Pan,Si-Si Luo,Jing Yan,Kai-Xuan Dong,Long-Yun Ye,Cheng-Liang Zhou,Jie-Xue Pan,Zhuo-Xian Meng,Ting Yu,Li Jin,Xian-Hua Lin,Yan-Ting Wu,Hong-Bo Yang,Xin-Mei Liu,Jian-Zhong Sheng,Guo-Lian Ding,He-Feng HuangPubMed 37914684
- Journal of translational medicine 21:3612023Dental pulp stem cell-derived exosomes revitalize salivary gland epithelial cell function in NOD mice via the GPER-mediated cAMP/PKA/CREB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShilin Hu,Bo Chen,Jiannan Zhou,Fangqi Liu,Tianjiao Mao,Janak L Pathak,Nobumoto Watanabe,Jiang LiPubMed 37268950
- Cancers 15:2023Characterization of Estrogen Receptors in Pancreatic Adenocarcinoma with Tertiary Lymphoid Structures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Zou,Yu Liu,Xuan Lin,Ruijie Wang,Zhengjie Dai,Yusheng Chen,Mingjian Ma,Yesiboli Tasiheng,Yu Yan,Xu Wang,Xianjun Yu,He Cheng,Chen LiuPubMed 36765788
- Cell death & disease 13:9152022Targeted activation of GPER enhances the efficacy of venetoclax by boosting leukemic pyroptosis and CD8+ T cell immune function in acute myeloid leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Ren,Yonghong Tao,Meixi Peng,Qiaoling Xiao,Yipei Jing,Junpeng Huang,Jing Yang,Can Lin,Minghui Sun,Li Lei,Zesong Yang,Zailin Yang,Ling ZhangPubMed 36316313
- Frontiers in cellular and infection microbiology 12:10372792022Sodium butyrate treatment and fecal microbiota transplantation provide relief from ulcerative colitis-induced prostate enlargement.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeimin Dong,Jiefang Zheng,Yiqiao Huang,Huijing Tan,Shengbang Yang,Zhiming Zhang,Xue Liang,Hao Liu,Guohao Zeng,Haoming Xu,Xianhan Jiang,Weide ZhongPubMed 36389141
- Journal of oncology 2022:24105302022GPR30 Activation Promotes the Progression of Gastric Cancer and Plays a Significant Role in the Anti-GC Effect of Huaier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Feng Wang,Can Hu,Shao-Wei Mo,Jing-Li Xu,Yan Liu,Han-Dong Xu,Li Yuan,Ling Huang,Jian-Fa Yu,Xiang-Dong Cheng,Zhi-Yuan XuPubMed 35096058
- Molecular biology reports 48:7853-78632021Saikosaponin-d alleviates hepatic fibrosis through regulating GPER1/autophagy signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYirong Chen,Renye Que,Na Zhang,Liubing Lin,Mengen Zhou,Yong LiPubMed 34714484
- Journal of inflammation research 14:843-8582021Estrogen Protects Articular Cartilage by Downregulating ASIC1a in Rheumatoid Arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Hang,Zhenyu Zhang,Ruowen Niu,Chen Wang,Jing Yao,Yayun Xu,Jingjing Tao,Lanlan Li,Feihu ChenPubMed 33737825
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.
Alternative names
CEPR, CMKRL2, DRY12, GPER, GPR30, GPER1, G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, G protein-coupled estrogen receptor 1, G-protein coupled receptor 30, GPCR-Br, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor, Membrane estrogen receptor, FEG-1, LYGPR, mER
Recommended products
Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033) is a rabbit monoclonal antibody detecting G-protein coupled receptor 30 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23701-79
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of G-protein coupled receptor 30?
Anti-G-protein coupled receptor 30 [EPR23701-79] (ab260033) specifically detects a band for G-protein coupled receptor 30 (UniProt: Q99527) at a molecular weight of 42kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR23701-79] also available for your convenience:
ab260033, Carrier free - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free ab275397, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab311048, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab311173, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab311828, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab313111, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab313304, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab321519
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--western-blot-img8100.jpg "Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)")
Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28849224, 29137421, 23273253).
Low expression control: A549 (PMID: 23273253).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 40 µg
Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 40 µg
Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 40 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99161.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling G-protein coupled receptor 30 with ab260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human placenta (PMID:9367686). The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76522.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on rat kidney PMID:21354433. The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120423.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)
Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse placenta. The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44698.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122762.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab26003 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-ab260033--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122762.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)")
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