Rabbit Recombinant Monoclonal G-protein coupled receptor 30 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--western-blot-img8100.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99161.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76522.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120423.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44698.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (AB275397)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Version
Target data
Function
G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.
Alternative names
G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, G protein-coupled estrogen receptor 1, G-protein coupled receptor 30, GPCR-Br, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor, Membrane estrogen receptor, FEG-1, LYGPR, mER, GPER1, GPR30, CEPR, DRY12, GPER, CMKRL2
Recommended products
Rabbit Recombinant Monoclonal G-protein coupled receptor 30 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Alternative names
G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, G protein-coupled estrogen receptor 1, G-protein coupled receptor 30, GPCR-Br, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor, Membrane estrogen receptor, FEG-1, LYGPR, mER, GPER1, GPR30, CEPR, DRY12, GPER, CMKRL2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23701-79
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab275397 is the carrier-free version of Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--western-blot-img8100.jpg "Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)")
Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)
This data was developed using Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28849224, 29137421, 23273253).
Low expression control: A549 (PMID: 23273253).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 40 µg
Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 40 µg
Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 40 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99161.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)
This data was developed using Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling G-protein coupled receptor 30 with Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human placenta (PMID:9367686). The section was incubated with Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76522.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)
This data was developed using Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on rat kidney PMID:21354433. The section was incubated with Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120423.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)
This data was developed using Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse placenta. The section was incubated with Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44698.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)
This data was developed using Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122762.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)
This data was developed using Anti-G-protein coupled receptor 30 antibody [EPR23701-79] ab260033, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab26003 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397), expandable thumbnail](https://content.abcam.com/products/images/g-protein-coupled-receptor-30-antibody-epr23701-79-bsa-and-azide-free-ab275397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122762.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] - BSA and Azide free (ab275397)")
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