Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling G3BP with purified ab181150 at 1/500 dilution (0.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

Immunohistochemical analysis of paraffin-embedded Human colon tissue staining G3BP using ab181150 (unpurified) at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells staining G3BP using ab181150 (unpurified) at 1/100 dilution, and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/200 dilution as a secondary antibody (red). Dapi counterstain (blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling G3BP with purified ab181150 at 1/30 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).

• IP

Supplier Data

Immunoprecipitation - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Western blot analysis of G3BP in immunoprecipitation pellets from Ramos cell lysate, using ab181151 (unpurified) at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-G3BP antibody [EPR13986(B)] (<a href='/en-us/products/primary-antibodies/g3bp-antibody-epr13986b-ab181150'>ab181150</a>) at 1/50 dilution

All lanes:

Immunoprecipitate from Ramos cell lysate using <a href='/en-us/products/primary-antibodies/g3bp-antibody-epr13986b-ab181150'>ab181150</a>

Secondary

All lanes:

Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

ab181150 (purified) at 1/20 dilution (2ug) immunoprecipitating G3BP in Ramos whole cell lysate. Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10ug
Lane 2 (+) : ab181150 & Ramos whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181150 in Ramos whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).

All lanes:

Immunoprecipitation - Anti-G3BP antibody [EPR13986(B)] (<a href='/en-us/products/primary-antibodies/g3bp-antibody-epr13986b-ab181150'>ab181150</a>)

Predicted band size: 52 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling G3BP with purified ab181150 at 1/50 dilution (6.86 μg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP antibody [EPR13986(B)] - BSA and Azide free (AB240247)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling G3BP with purified ab181150 at 1/50 dilution (6.86 μg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181150).