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Rabbit Recombinant Monoclonal G3BP2 antibody. Carrier free. Suitable for Dot, IHC-P, Flow Cyt (Intra), WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (AB317688), expandable thumbnail
  • Dot Blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (AB317688), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (AB317688), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (AB317688), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (AB317688), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-PFlow Cyt (Intra)WBICC/IFIP
Human
Expected
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Expected
Tested
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling (PubMed:23279204, PubMed:32302570, PubMed:32302571, PubMed:32302572). Plays an essential role in stress granule formation (PubMed:27022092, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:35977029). Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress (PubMed:32302570, PubMed:32302571, PubMed:32302572). Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal G3BP2 antibody. Carrier free. Suitable for Dot, IHC-P, Flow Cyt (Intra), WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27325-43
Purification technique
Affinity purification Protein A
Specificity

This antibody specifically recognizes G3BP2 (isoform a) but not G3BP2 (isoform b).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317688 is the carrier-free version of Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    The bands beneath the target band (40 kDa) are likely to be degraded target fragments.

    Exposure time: Lanes 1-6: 10 seconds, Lanes 7-11: 48 seconds

    All lanes: Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] (Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687) at 1/1000 dilution

    Lanes 1 and 9: Mouse brain tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Mouse liver tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Mouse testis tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Rat brain tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Rat liver tissue lysate at 20 µg with NFDM/TBST

    Lane 6: Rat testis tissue lysate at 20 µg with NFDM/TBST

    Lane 7: Human cerebellum tissue lysate at 20 µg with NFDM/TBST

    Lane 8: Mouse cerebral cortex tissue lysate at 20 µg with NFDM/TBST

    Lane 10: Rat cerebellum tissue lysate at 20 µg with NFDM/TBST

    Lane 11: Rat cerebral cortex tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 70 kDa, 40 kDa

  • Dot Blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Dot Blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    Dot blot analysis of G3BP2 (isoform a) using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 at 1:1000 (0.527 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane 1:His-tagged human G3BP2 fragment
    Lane 2:His-tagged human G3BP1 fragment

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human G3BP1.

    Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) as total protein control staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] (Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687) at 1/1000 dilution

    Lane 1: His-tagged human G3BP2 recombinant protein

    Lane 2: His-tagged human G3BP1 recombinant protein

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling G3BP2 (isoform a) with Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on neurons of rat cerebrum. The section was incubated with Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling G3BP2 (isoform a) with Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on neurons of mouse cerebrum. The section was incubated with Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling G3BP2 (isoform a) with Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 at 1/100 (5.27 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on neurons of human cerebrum (PMID: 33203845). The section was incubated with Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling G3BP2 (isoform a) with Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] (Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 70 kDa

    Exposure time: 26s

  • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688), expandable thumbnail

    Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    This data was developed using Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] (Anti-G3BP2 (isoform a) antibody [EPR27325-43] ab317687) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting G3BP2 whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 70 kDa, 124 kDa

    Exposure time: 81s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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