Rabbit Recombinant Monoclonal GAA antibody. Carrier free. Suitable for WB, IP and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--western-blot-img421012.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449)")
![Immunoprecipitation - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--immunoprecipitation-img421052.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449)")
![Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--western-blot-img421014.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449)")
![Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--western-blot-img421013.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (AB314449)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans.
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Gaa
Recommended products
Rabbit Recombinant Monoclonal GAA antibody. Carrier free. Suitable for WB, IP and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28363-56
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314449 is the carrier-free version of Anti-GAA antibody [EPR28363-56] ab314448.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--western-blot-img421012.jpg "Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)")
Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)
This data was developed using Anti-GAA antibody [EPR28363-56] ab314448, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 33971197).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GAA antibody [EPR28363-56] (Anti-GAA antibody [EPR28363-56] ab314448) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: NIH/3T3 transfected with siRNA specifically targeti GAA whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 76 kDa, 95 kDa, 110 kDa
Exposure time: 180s
![Immunoprecipitation - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--immunoprecipitation-img421052.jpg "Immunoprecipitation - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)")
Immunoprecipitation - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)
This data was developed using Anti-GAA antibody [EPR28363-56] ab314448, the same antibody clone in a different buffer formulation.
GAA was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-GAA antibody [EPR28363-56] ab314448 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GAA antibody [EPR28363-56] ab314448 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: Anti-GAA antibody [EPR28363-56] ab314448 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GAA antibody [EPR28363-56] ab314448 NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-GAA antibody [EPR28363-56] (Anti-GAA antibody [EPR28363-56] ab314448) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--western-blot-img421014.jpg "Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)")
Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)
This data was developed using Anti-GAA antibody [EPR28363-56] ab314448, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 33971197).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-GAA antibody [EPR28363-56] (Anti-GAA antibody [EPR28363-56] ab314448) at 1/1000 dilution
Lane 1: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 3: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 76 kDa, 95 kDa, 110 kDa
Exposure time: 26s
![Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449), expandable thumbnail](https://content.abcam.com/products/images/gaa-antibody-epr28363-56-bsa-and-azide-free-ab314449--western-blot-img421013.jpg "Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)")
Western blot - Anti-GAA antibody [EPR28363-56] - BSA and Azide free (ab314449)
This data was developed using Anti-GAA antibody [EPR28363-56] ab314448, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 33971197).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-GAA antibody [EPR28363-56] (Anti-GAA antibody [EPR28363-56] ab314448) at 1/1000 dilution
Lane 1: Mouse heart tissue lysate at 20 µg
Lane 2: Mouse thymus tissue lysate at 20 µg
Lane 3: Mouse cerebellum tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat cerebellum tissue lysate at 20 µg
Lane 6: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 76 kDa, 95 kDa, 110 kDa
Exposure time: 180s
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