AB240102

Anti-GAA antibody [EPR4716(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GAA antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA

3 Images

Supplier Data

Western blot - Anti-GAA antibody [EPR4716(2)] - BSA and Azide free (AB240102)

This data was developed using ab137068, the same antibody clone in a different buffer formulation.

Exposure time : Lane 1 : 10 seconds; Lanes 2-3 : 30 seconds.

All lanes:

Western blot - Anti-GAA antibody [EPR4716(2)] (<a href='/en-us/products/primary-antibodies/gaa-antibody-epr47162-ab137068'>ab137068</a>) at 1/1000 dilution

Lane 1:

Human liver lysates at 15 µg

Lane 2:

Human brain lysates at 15 µg

Lane 3:

Human thymus lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 105 kDa

Observed band size: 110 kDa,75 kDa,95 kDa

false

Unknown

Western blot - Anti-GAA antibody [EPR4716(2)] - BSA and Azide free (AB240102)

This data was developed using ab137068, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GAA antibody [EPR4716(2)] (<a href='/en-us/products/primary-antibodies/gaa-antibody-epr47162-ab137068'>ab137068</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

Human placenta tissue lysate at 10 µg

Predicted band size: 105 kDa

Observed band size: 110 kDa,75 kDa,95 kDa

false

Lab

Western blot - Anti-GAA antibody [EPR4716(2)] - BSA and Azide free (AB240102)

This data was developed using ab137068, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GAA antibody [EPR4716(2)] (<a href='/en-us/products/primary-antibodies/gaa-antibody-epr47162-ab137068'>ab137068</a>) at 1/1000 dilution

All lanes:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 105 kDa

false

Exposure time: 20s

Unconjugated

Anti-GAA antibody [EPR4716(2)]
HRP

HRP Anti-GAA antibody [EPR4716(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4716(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240102 is the carrier-free version of ab137068.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the degradation of glycogen in lysosomes (PubMed : 14695532, PubMed : 18429042, PubMed : 1856189, PubMed : 7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed : 29061980).

See full target information Lysosomal alpha-glucosidase

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:790-798 PubMed40533564

2025

Kupffer cell programming by maternal obesity triggers fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Huang,Nora R Balzer,Lea Seep,Iva Splichalova,Nelli Blank-Stein,Maria Francesca Viola,Eliana Franco Taveras,Kerim Acil,Diana Fink,Franzisca Petrovic,Nikola Makdissi,Seyhmus Bayar,Katharina Mauel,Carolin Radwaniak,Jelena Zurkovic,Amir H Kayvanjoo,Klaus Wunderling,Malin Jessen,Mohamed H Yaghmour,Lukas Kenner,Thomas Ulas,Stephan Grein,Joachim L Schultze,Charlotte L Scott,Martin Guilliams,Zhaoyuan Liu,Florent Ginhoux,Marc D Beyer,Christoph Thiele,Felix Meissner,Jan Hasenauer,Dagmar Wachten,Elvira Mass

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAA antibody [EPR4716(2)] - BSA and Azide free

Western blot - Anti-GAA antibody [EPR4716(2)] - BSA and Azide free (AB240102)

Western blot - Anti-GAA antibody [EPR4716(2)] - BSA and Azide free (AB240102)

Western blot - Anti-GAA antibody [EPR4716(2)] - BSA and Azide free (AB240102)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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Product promise