Rabbit Polyclonal GAA1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GPAA1 aa 200-500.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GPAA1 aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link O43292
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Component of the GPI transamidase complex, necessary for transfer of GPI to proteins (PubMed:34576938). Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
Alternative names
GAA1, GPAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPI anchor attachment protein 1, GAA1 protein homolog, hGAA1
Recommended products
Rabbit Polyclonal GAA1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GPAA1 aa 200-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GPAA1 aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link O43292
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-GAA1 antibody (ab126951)
7.5% SDS Page
All lanes: Western blot - Anti-GAA1 antibody (ab126951) at 1/1000 dilution
All lanes: HepG2 cell lysate at 30 µg
Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAA1 antibody (ab126951)
ab126951, at a 1/500 dilution, staining GAA1 in paraffin-embedded Human hepatoma tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAA1 antibody (ab126951)
ab126951, at a 1/500 dilution, staining GAA1 in paraffin-embedded Mouse muscle tissue by immunohistochemistry.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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