Knockout Tested Rabbit Recombinant Monoclonal GAB1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-GAB1 antibody [EPR26118-37] (AB319136), expandable thumbnail](https://content.abcam.com/products/images/gab1-antibody-epr26118-37-ab319136--western-blot-img434800.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAB1 antibody [EPR26118-37] (AB319136)")
![Western blot - Anti-GAB1 antibody [EPR26118-37] (AB319136), expandable thumbnail](https://content.abcam.com/products/images/gab1-antibody-epr26118-37-ab319136--western-blot-img434799.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAB1 antibody [EPR26118-37] (AB319136)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed:29408807).
Alternative names
GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, GAB1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal GAB1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26118-37
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136), expandable thumbnail](https://content.abcam.com/products/images/gab1-antibody-epr26118-37-ab319136--western-blot-img434800.jpg "Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136)")
Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1: 37 seconds; Lane 2: 180 seconds.
All lanes: Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 30 µg
Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 100 kDa
![Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136), expandable thumbnail](https://content.abcam.com/products/images/gab1-antibody-epr26118-37-ab319136--western-blot-img434799.jpg "Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136)")
Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, ab319136 was shown to bind specifically to GAB1. Target of interest was observed at 100 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in GAB1 knockout cell line (lane 2).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GAB1 antibody [EPR26118-37] (ab319136) at 1/1000 dilution
Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 30 µg
Lane 2: GAB1 knockout HAP1 whole cell lysate at 30 µg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 30 µg
Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 30 µg
Lane 5: Mouse brain tissue lysate at 30 µg
Lane 6: Mouse lung tissue lysate at 30 µg
Lane 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 8: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 100 kDa, 36 kDa
Exposure time: 136s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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