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Rabbit Recombinant Monoclonal GAB1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.

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Images

Western blot - Anti-GAB1 antibody [EPR375] (AB133486), expandable thumbnail
  • Western blot - Anti-GAB1 antibody [EPR375] (AB133486), expandable thumbnail
  • Immunoprecipitation - Anti-GAB1 antibody [EPR375] (AB133486), expandable thumbnail
  • Western blot - Anti-GAB1 antibody [EPR375] (AB133486), expandable thumbnail
  • Western blot - Anti-GAB1 antibody [EPR375] (AB133486), expandable thumbnail

Publications

  • Neuro-oncology 25:899-9122022
    Epigenetic upregulation of Schlafen11 renders 
WNT- and SHH-activated medulloblastomas sensitive to cisplatin.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Satoshi Nakata,Junko Murai,Masayasu Okada,Haruhiko Takahashi,Tyler H Findlay,Kristen Malebranche,Akhila Parthasarathy,Satoshi Miyashita,Ramil Gabdulkhaev,Ilan Benkimoun,Sabine Druillennec,Sara Chabi,Eleanor Hawkins,Hiroaki Miyahara,Kensuke Tateishi,Shinji Yamashita,Shiori Yamada,Taiki Saito,Jotaro On,Jun Watanabe,Yoshihiro Tsukamoto,Junichi Yoshimura,Makoto Oishi,Toshimichi Nakano,Masaru Imamura,Chihaya Imai,Tetsuya Yamamoto,Hideo Takeshima,Atsuo T Sasaki,Fausto J Rodriguez,Sumihito Nobusawa,Pascale Varlet,Celio Pouponnot,Satoru Osuka,Yves Pommier,Akiyoshi Kakita,Yukihiko Fujii,Eric H Raabe,Charles G Eberhart,Manabu Natsumeda
    PubMed 36273330
  • Molecular medicine reports 26:2022
    Interference of PTK6/GAB1 signaling inhibits cell proliferation, invasion, and migration of cervical cancer cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Juan Li,Nan Yang,Xiaolei Tian,Linglong Ouyang,Man Jiang,Shufang Zhang
    PubMed 35894144

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed:29408807).

      Alternative names

      GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, GAB1

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      Rabbit Recombinant Monoclonal GAB1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.

      Alternative names

      GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, GAB1

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR375

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Western blot - Anti-GAB1 antibody [EPR375] (ab133486), expandable thumbnail

        Western blot - Anti-GAB1 antibody [EPR375] (ab133486)

        Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
        Lane 2: GAB1 knockout HAP1 whole cell lysate (20 μg)
        Lane 3: Hek293 whole cell lysate (20 μg)
        Lane 4: HepG2 whole cell lysate (20 μg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab133486 observed at 100 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

        ab133486 was shown to recognize GAB1 in wild-type HAP1 cells as signal was lost at the expected MW in GAB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GAB1 knockout samples were subjected to SDS-PAGE. ab133486 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-GAB1 antibody [EPR375] (ab133486)

        Predicted band size: 77 kDa

      • Western blot - Anti-GAB1 antibody [EPR375] (ab133486), expandable thumbnail

        Western blot - Anti-GAB1 antibody [EPR375] (ab133486)

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

        All lanes: Western blot - Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution

        All lanes: K562 cell lysate at 10 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

        Predicted band size: 77 kDa

      • Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486), expandable thumbnail

        Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486)

        ab133486 (purified) at 1/80 immunoprecipitating GAB1 in HEK293 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used intead of Anti-Rab11A antibody [EPR7587(B)] ab128913 as a negative control (Lane 3).

        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.

        All lanes: Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486)

        Predicted band size: 77 kDa

      • Western blot - Anti-GAB1 antibody [EPR375] (ab133486), expandable thumbnail

        Western blot - Anti-GAB1 antibody [EPR375] (ab133486)

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

        All lanes: Western blot - Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution

        All lanes: HEK293 cell lysate at 10 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

        Predicted band size: 77 kDa

      • Western blot - Anti-GAB1 antibody [EPR375] (ab133486), expandable thumbnail

        Western blot - Anti-GAB1 antibody [EPR375] (ab133486)

        All lanes: Western blot - Anti-GAB1 antibody [EPR375] (ab133486) at 1/1000 dilution

        Lane 1: HepG2 cell lysate at 10 µg

        Lane 2: K562 cell lysate at 10 µg

        Lane 3: 293T cell lysate at 10 µg

        Lane 4: SH-SY5Y cell lysate at 10 µg

        Secondary

        All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 77 kDa

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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