AB32365

Anti-GAB2 antibody [EP369Y]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal GAB2 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

KIAA0571, GAB2, GRB2-associated-binding protein 2, GRB2-associated binder 2, Growth factor receptor bound protein 2-associated protein 2, pp100

1 Images

Lab

Western blot - Anti-GAB2 antibody [EP369Y] (AB32365)

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

Blocking/Diluting Buffer and concentration :
5% NFDM/TBST

All lanes:

Western blot - Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in RIPA lysis method at 15 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in 1% SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 97 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP369Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is predicted to detect the splice isoform 2 based on sequence homology.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

See full target information GAB2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutical biology 59:672-682 PubMed34078224

2021

Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbin Sun,Sixing Huang,Yao Qin,Ping Zhang,Ziwei Li,Li Zhang,Xin Wang,Ruijun Wu,Shaorong Qin,Jiayong Huo,Kunquan Xiao,Weizao Luo

Oncology reports 44:1961-1970 PubMed32901879

2020

MicroRNA‑218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Mu,Bing Guan,Juanhua Tian,Xiang Li,Qingzhi Long,Meiyu Wang,Wen Wang,Junjun She,Xudong Li,Dapeng Wu,Yuefeng Du

Molecular medicine reports 13:4279-88 PubMed27035789

2016

MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma.

Applications

Species

Unspecified reactive species

Li-Qiang Tian,En-Qin Liu,Xi-De Zhu,Xin-Gong Wang,Jian Li,Guang-Ming Xu

The Journal of biological chemistry 283:23505-9 PubMed18586679

2008

Interleukin-3 (IL-3)-induced c-fos activation is modulated by Gab2-calcineurin interaction.

Applications

Species

Unspecified reactive species

Saiju Pyarajan,Gabriel Matejovic,Joanne C Pratt,Shairaz Baksh,Steven J Burakoff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAB2 antibody [EP369Y]

Western blot - Anti-GAB2 antibody [EP369Y] (AB32365)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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