AB151573

Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal GABA A Receptor alpha 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1, GABAAR subunit alpha-1, GABRA1

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151573 showing -ve staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151573 showing -ve staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling GABA A Receptor alpha 1 with Purified ab151573 at 1 : 1000 dilution (0.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab151573 showing -ve staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151573 showing -ve staining.

Unknown

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Blocking and diluting buffer : 5% NFDM/TBST.

Please note : Lysates prepared in 1%SDS Hot lysis method.

According to the paper (PMID : 29467616), brain is positive and lung is negative tissue.

All lanes:

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 0.7 µg/mL

Lane 1:

Human brain lysates at 15 µg

Lane 2:

Human lung lysates - negative control at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 52 kDa

false

Lab

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

All lanes:

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution

Lane 1:

Human brain prepared in RIPA lysis method at 15 µg

Lane 2:

Human brain prepared in 1%SDS Hot lysis method at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 40s

Unknown

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Please note : Lysates prepared in 1%SDS Hot lysis method.

All lanes:

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

false

Carrier free

Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5401(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "We recommend to try 1% SDS Hot lysis prepare method to get desired WB results.For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "For unpurified use 1/50 - 1/100 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by Gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 23909897, PubMed : 25489750, PubMed : 29950725, PubMed : 30602789). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed : 29950725, PubMed : 30602789). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 23909897, PubMed : 29950725, PubMed : 30602789). Alpha-1/GABRA1-containing GABAARs are largely synaptic (By similarity). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (PubMed : 23909897, PubMed : 25489750). GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response (By similarity). GABAARs containing alpha, beta and epsilon subunits also permit spontaneous chloride channel activity while preserving the structural information required for GABA-gated openings (By similarity). Alpha-1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (By similarity). Together with rho subunits, may also control neuronal and glial GABAergic transmission in the cerebellum (By similarity).

See full target information GABRA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 30: PubMed38785151

2024

Oroxylin A inhibits inflammatory cytokines in periodontitis via HO‑1.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Wang,Zhao-Bo Wang,Chun-Miao Jiang,Yang Zhao,Li Tang,Xi-Mei Xiao,Jing Fu

Scientific reports 9:4512 PubMed30872728

2019

Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sidra Tabassum,Afzal Misrani,Bin-Liang Tang,Jian Chen,Li Yang,Cheng Long

The Journal of comparative neurology 526:803-823 PubMed29218727

2017

GABA and GABA receptor subunit localization on neurochemically identified neurons of the human subthalamic nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Hua Wu,Jennifer Junru Song,Richard Lewis Maxwell Faull,Henry John Waldvogel

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (AB151573)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise