AB216973

Anti-GABA A Receptor alpha 2/GABRA2 antibody

Name: Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab216973) | Abcam
Brand: Abcam Limited
SKU: AB216973
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GABA A Receptor alpha 2/GABRA2 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GABRA2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2, GABAAR subunit alpha-2, GABRA2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Rat brain tissue labeling GABA A Receptor alpha 2/GABRA2 with ab216973 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry (Intracellular) - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

Intracellular flow cytometric analysis of RSC96 cells labeling GABA A Receptor alpha 2/GABRA2 with ab216973 at 1/100 dilution followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Supplier Data

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

All lanes:

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab216973) at 1/300 dilution

All lanes:

Mouse brain cell lysate

Secondary

All lanes:

conjugated secondary at 1/5000 dilution

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human GABRA2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P47869

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 10449790, PubMed : 29961870, PubMed : 31032849). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interfaces (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 10449790). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). The alpha-2 subunit exhibits synaptogenic activity together with beta-2 and very little to no activity together with beta-3, the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (By similarity).

See full target information GABRA2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70197 PubMed39749638

2025

N-Demethylsinomenine Relieves Neuropathic Pain in Male Mice Mainly via Regulating α2-Subtype GABA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Rong,Xunjia Qian,Yujian Yin,Yipeng Gu,Weiyi Su,Jie-Jia Li,Yue Xu,Hongyan Zhu,Junxu Li,Qing Zhu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2307747 PubMed38896791

2024

Sequential Inhibition of PARP and BET as a Rational Therapeutic Strategy for Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Peng,Xin Huang,Shaolu Zhang,Naixin Zhang,Shengfan Huang,Yingying Wang,Zhenxing Zhong,Shan Zhu,Haiwang Gao,Zixiang Yu,Xiaotong Yan,Zhennan Tao,Yuxiang Dai,Zhe Zhang,Xi Chen,Feng Wang,Francois X Claret,Moshe Elkabets,Ning Ji,Yuxu Zhong,Dexin Kong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA A Receptor alpha 2/GABRA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

Flow Cytometry (Intracellular) - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody (AB216973)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise