Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat hippocampal neuron cells labelling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/100 (4.81 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in rat hippocampal neuron. Confocal scanning Z step was set as 0.3 ¦Мm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/100 (4.81 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 ¦Мm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/2000 (0.241 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat hippocampus (PMID : 23337532). The section was incubated with ab307359 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue lABeling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/2000 (0.241 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on mouse lung. The section was incubated with ab307359 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse hippocampus (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/100 (4.81 ug/ml) dilution followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse lung (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/100 (4.81 ug/ml) dilution followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression : confocal image showing no staining on mouse lung (PMID : 29467616). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue lABeling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/2000 (0.241 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Positive staining on mouse hippocampus (PMID : 23337532). The section was incubated with ab307359 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat lung (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/100 (4.81 ug/ml) dilution followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression : confocal image showing no staining on rat lung (PMID : 29467616). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat hippocampus (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with ab307359 at 1/100 (4.81 ug/ml) dilution followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor®, 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount®,.The immunostaining was performed on a Leica Biosystems BOND®, RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor®, 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IP

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Immunoprecipitation - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

GABA A Receptor alpha 2/GABRA2 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate 10 ug with ab307359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse cerebellum tissue lysate 10 ug Lane 2 : ab307359 IP in Mouse cerebellum tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307359 in mouse cerebellum tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (ab307359) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate 10 μg

Lane 2:

Mouse cerebellum tissue lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab307359 in mouse cerebellum tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 51 kDa

false

Exposure time: 24s

• WB

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Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTSamples are non-boiled as boiling may cause protein aggregates. Exposure time : 48 seconds

All lanes:

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (ab307359) at 1/1000 dilution

All lanes:

Human hippocampus tissue lysate 40 μg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 51 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLow expression : liver, lung (PMID : 29467616) Samples are non-boiled as boiling may cause protein aggregates. Exposure time : 48 seconds

All lanes:

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (ab307359) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate 80 μg

Lane 2:

Mouse brain tissue lysate 80 μg

Lane 3:

Mouse lu tissue lysate 80 μg

Lane 4:

Mouse liver tissue lysate 80 μg

Lane 5:

Rat cerebellum tissue lysate 80 μg

Lane 6:

Rat brain tissue lysate 80 μg

Lane 7:

Rat lu tissue lysate 80 μg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 51 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (AB307359)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTSamples are non-boiled as boiling may cause protein aggregates. Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (ab307359) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate 20 μg

Lane 2:

Rat hippocampus tissue lysate 20 μg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 51 kDa

false

Exposure time: 5.5s