Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free

13 product images

• 

  Immunoprecipitation - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  GABA A Receptor alpha 2/GABRA2 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate 10 ug with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: Mouse cerebellum tissue lysate 10 ug
  Lane 2: Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 IP in Mouse cerebellum tissue lysate
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 in mouse cerebellum tissue lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 24 seconds

  All lanes: Immunoprecipitation - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359) at 1/1000 dilution

  Lane 1: Mouse cerebellum tissue lysate 10 μg

  Lane 2: Mouse cerebellum tissue lysate

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 in mouse cerebellum tissue lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 51 kDa

  Exposure time: 24s

• 

  Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using 307359, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Samples are non-boiled as boiling may cause protein aggregates.
  5.5 seconds
  Exposure time:

  All lanes: Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359) at 1/1000 dilution

  Lane 1: Mouse hippocampus tissue lysate 20 μg

  Lane 2: Rat hippocampus tissue lysate 20 μg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 51 kDa

  Exposure time: 5.5s

• 

  Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using 307359, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Low expression: liver, lung (PMID:29467616)
  
  Samples are non-boiled as boiling may cause protein aggregates.
  48 seconds
  Exposure time:

  All lanes: Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359) at 1/1000 dilution

  Lane 1: Mouse cerebellum tissue lysate 80 μg

  Lane 2: Mouse brain tissue lysate 80 μg

  Lane 3: Mouse lu tissue lysate 80 μg

  Lane 4: Mouse liver tissue lysate 80 μg

  Lane 5: Rat cerebellum tissue lysate 80 μg

  Lane 6: Rat brain tissue lysate 80 μg

  Lane 7: Rat lu tissue lysate 80 μg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 51 kDa

  Exposure time: 48s

• 

  Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using 307359, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Samples are non-boiled as boiling may cause protein aggregates.
  48 seconds
  Exposure time:

  All lanes: Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] (Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359) at 1/1000 dilution

  All lanes: Human hippocampus tissue lysate 40 μg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 51 kDa

  Exposure time: 48s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/2000 (0.241 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat hippocampus (PMID: 23337532). The section was incubated with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/2000 (0.241 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: No staining on mouse lung. The section was incubated withab307359 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/2000 (0.241 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse hippocampus (PMID: 23337532). The section was incubated with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat lung (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/100 (4.81 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression: confocal image showing no staining on rat lung (PMID: 29467616). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat hippocampus (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/100 (4.81 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse lung (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/100 (4.81 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression: confocal image showing no staining on mouse lung (PMID: 29467616). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse hippocampus (fresh) tissue lABeling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/100 (4.81 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized rat hippocampal neuron cells lABelling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/100 (4.81 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in rat hippocampal neuron.Confocal scanning Z step was set as 0.3 ¦Мm followed by image processing with maximum Z projection.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/ml dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] - BSA and Azide free (ab307360)

  This data was developed using Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359, the same antibody clone in a different buffer formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized mouse primary neuron cells lABelling GABA A Receptor alpha 2/GABRA2 with Anti-GABA A Receptor alpha 2/GABRA2 antibody [EPR26485-185] ab307359 at 1/100 (4.81 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 ¦Мm followed by image processing with maximum Z projection.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/ml dilution.