Rabbit Polyclonal GABA A Receptor alpha 3/GABRA3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GABRA3 aa 1-150.
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Publications
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- Molecular brain 14:962021Reductions in midbrain GABAergic and dopamine neuron markers are linked in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTertia D Purves-Tyson,Amelia M Brown,Christin Weissleder,Debora A Rothmond,Cynthia Shannon WeickertPubMed 34174930
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GABRA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P34903
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Alternative names
Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3, GABRA3
Recommended products
Rabbit Polyclonal GABA A Receptor alpha 3/GABRA3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GABRA3 aa 1-150.
Alternative names
Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3, GABRA3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GABRA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P34903
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
Paraffin-embedded human duodenum tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
Paraffin-embedded human cerebral cortex tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
Paraffin-embedded mouse ventral pallidum tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
Paraffin-embedded mouse olfactory bulb tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
Paraffin-embedded mouse insular cortex tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
Paraffin-embedded mouse thalamus tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
All lanes: Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214) at 1/250 dilution
All lanes: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 55 kDa
Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214)
All lanes: Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214) at 1/250 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 55 kDa
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