AB224214

Anti-GABA A Receptor alpha 3/GABRA3 antibody

Name: Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214) | Abcam
Brand: Abcam Limited
SKU: AB224214
Price: 545 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal GABA A Receptor alpha 3/GABRA3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABRA3 aa 1-150.

View Alternative Names

Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3, GABAAR subunit alpha-3, GABRA3

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded human cerebral cortex tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded human duodenum tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse thalamus tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse ventral pallidum tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse olfactory bulb tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse insular cortex tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Supplier Data

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

All lanes:

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214) at 1/250 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate

Predicted band size: 55 kDa

true

Supplier Data

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

All lanes:

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 55 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GABRA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P34903

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 16412217, PubMed : 29053855). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 16412217, PubMed : 29053855). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (PubMed : 16412217, PubMed : 29053855).

See full target information GABRA3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of neural transmission (Vienna, Austria : 1996) : PubMed40886229

2025

The effect of recombinant botulinum neurotoxin A on neuropathic pain in the spared nerve injury mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasmus E Hammer,Akinyemi A Omoniyi,Mette Richner,Stephane Lezmi,Christian B Vaegter,Mikhail Kalinichev,Pall Karlsson,Jens R Nyengaard

PloS one 19:e0296024 PubMed38206981

2024

Expression of the cobalamin transporters cubam and MRP1 in the canine ileum-Upregulation in chronic inflammatory enteropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Kather,Johannes Kacza,Helga Pfannkuche,Denny Böttcher,Chi-Hsuan Sung,Joerg M Steiner,Gotthold Gäbel,Franziska Dengler,Romy M Heilmann

Molecular brain 14:96 PubMed34174930

2021

Reductions in midbrain GABAergic and dopamine neuron markers are linked in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Tertia D Purves-Tyson,Amelia M Brown,Christin Weissleder,Debora A Rothmond,Cynthia Shannon Weickert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA A Receptor alpha 3/GABRA3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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