Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)

Rabbit Recombinant Monoclonal GABA A Receptor alpha 3/GABRA3 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (AB316970), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	IP	ICC/IF	Flow Cyt
Human	Tested	Tested	Expected	Tested	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Expected	Not recommended	Not recommended
Rat	Tested	Tested	Tested	Expected	Not recommended	Not recommended
Transfected cell lysate - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Target data

See full target information GABRA3

Function

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:16412217, PubMed:29053855). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed:16412217, PubMed:29053855). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (PubMed:16412217, PubMed:29053855).

Alternative names

Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3, GABAAR subunit alpha-3, GABRA3

Recommended products

Rabbit Recombinant Monoclonal GABA A Receptor alpha 3/GABRA3 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28364-82
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab316970 is the carrier-free version of Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver (PMID: 26869349). The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Negative control: liver (PMID: 26869349), spleen (PMID: 26869349).
In lanes 1-9, the lysates were stored at -80°C prior to Western Blotting. The bands beneath the target band (56 kDa) are likey to be degradation products. In lanes 10-11, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
Lanes 1-4 use Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity to human IgG at 1/2000. Lanes 5-11 use Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-4: 3 seconds, lanes 5-7: 15 seconds, lanes 8-9: 3 seconds, lanes 10-11: 59 seconds
All lanes: Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] (Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 20 µg with NFDM/TBST
Lane 2: Human hypothalamus tissue lysate at 20 µg with NFDM/TBST
Lane 3: Human liver tissue lysate at 20 µg with NFDM/TBST
Lane 4: Human spleen tissue lysate at 20 µg with NFDM/TBST
Lanes 5 and 10: Mouse brain tissue lysate at 20 µg with NFDM/TBST
Lane 6: Mouse cerebellum tissue lysate at 20 µg with NFDM/TBST
Lane 7: Mouse spleen tissue lysate at 20 µg with NFDM/TBST
Lanes 8 and 11: Rat brain tissue lysate at 20 µg with NFDM/TBST
Lane 9: Rat spleen tissue lysate at 20 µg with NFDM/TBST
Secondary
Lanes 1 - 4: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 5 - 11: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 56 kDa, 36 kDa
Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Low expression: MCF7 (PMID: 26869349), HepG2.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28472809).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] (Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969) at 1/1000 dilution
Lane 1: SW1353 (human chondrosarcoma fibroblast) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 56 kDa, 36 kDa
Exposure time: 48s
Immunoprecipitation - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
GABA A Receptor alpha 3/GABRA3 was immunoprecipitated from 0.35 mg SW1353 (human chondrosarcoma fibroblast) whole cell lysate with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SW1353 (human chondrosarcoma fibroblast) whole cell lysate

Lane 2: Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 IP in SW1353 (human chondrosarcoma fibroblast) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 in SW1353 whole cell lysate
The band at approximately 50KDa may be a degradation/cleavage product.
All lanes: Immunoprecipitation - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] (Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969) at 1/30 dilution
All lanes: SW1353 (human chondrosarcoma fibroblast) whole cell lysate at 10 µg with NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with human GBRA1, GBRA2, GBRA4, GBRA5, GBRA6.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-Myc tag antibody staining at 1/1000 dilution.
All lanes: Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] (Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Lane 2: 293T transfected with a human GBRA1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Lane 3: 293T transfected with a human GBRA2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Lane 4: 293T transfected with a human GBRA3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Lane 5: 293T transfected with a human GBRA4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Lane 6: 293T transfected with a human GBRA5 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Lane 7: 293T transfected with a human GBRA6 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 56 kDa, 15 kDa
Exposure time: 180s
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh frozen) tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/100 (4.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on rat spleen. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/100 (4.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Panel A: merged staining of anti-GABRA3 (Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on rat cerebrum.

Panel B: anti-GABRA3 stained on rat cerebrum.

Panel C: anti-NeuN stained in neurons of rat cerebrum.

Panel D: anti-GFAP stained in astrocytes of rat cerebrum.

The section was incubated in two rounds of staining: in the order of Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh frozen) tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/100 (4.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on mouse spleen. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/100 (4.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Panel A: merged staining of anti-GABRA3 (Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on mouse cerebrum.

Panel B: anti-GABRA3 stained on mouse cerebrum.

Panel C: anti-NeuN stained in neurons of mouse cerebrum.

Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.

The section was incubated in two rounds of staining: in the order of Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human spleen (PMID: 26869349). The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum. The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat spleen. The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse spleen. The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] - BSA and Azide free (ab316970)
This data was developed using Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GABA A Receptor alpha 3/GABRA3 with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum (PMID: 26869349). The section was incubated with Anti-GABA A Receptor alpha 3/GABRA3 antibody [EPR28364-82] ab316969 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins